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Citations & References (5)
Invitrogen™
DNP-X, SE, 6-(2,4-Dinitrophenyl)aminohexanoic Acid, Succinimidyl Ester
The amine-reactive DNP-X succinimidyl ester can be used bioconjugates that can be detected with anti-dinitrophenyl (anti-DNP) antibodies. This reactive haptenRead more
| Catalog Number | Quantity |
|---|---|
| D2248 also known as D-2248 | 25 mg |
Catalog number D2248
also known as D-2248
Price (HKD)
2,171.00
Each
Quantity:
25 mg
Price (HKD)
2,171.00
Each
The amine-reactive DNP-X succinimidyl ester can be used bioconjugates that can be detected with anti-dinitrophenyl (anti-DNP) antibodies. This reactive hapten contains an additional seven-atom aminohexanoyl spacer ('X') between the fluorophore and the succinimidyl ester group. This spacer helps to separate increase accessibility to anti-DNP antibodies.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Label or DyeDNP
Product TypeDNP-X Succinimidyl Ester
Quantity25 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
Label TypeBiotin and Other Haptens
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (5)
Citations & References
Abstract
Endosomal density shift is related to a decrease in fusion capacity.
Journal:Eur J Cell Biol
PubMed ID:1802709
'Dinitrophenol (DNP)-beta-glucuronidase and mannosylated anti-DNP IgG, which are endocytosed by the mannose receptor and delivered to lysosomes, were previously developed as probes for examination of fusion between early endosomes in a cell-free system. In this study, these probes were found to be transported by intact cells to endocytic vesicles with
Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.
Journal:Cytometry A
PubMed ID:20824632
'Intracellular flow cytometry permits quantitation of diverse molecular targets at the single-cell level. However, limitations in detection sensitivity inherently restrict the method, sometimes resulting in the inability to measure proteins of very low abundance or to differentiate cells expressing subtly different protein concentrations. To improve these measurements, an enzymatic amplification
Impact of hapten presentation on antibody binding at lipid membrane interfaces.
Journal:Biophys J
PubMed ID:18199665
We report the effects of ligand presentation on the binding of aqueous proteins to solid supported lipid bilayers. Specifically, we show that the equilibrium dissociation constant can be strongly affected by ligand lipophilicity and linker length/structure. The apparent equilibrium dissociation constants (K(D)) were compared for two model systems, biotin/anti-biotin and
IgE-mediated activation of NK cells through Fc gamma RIII.
Journal:J Immunol
PubMed ID:12626560
NK cells express Fc gamma RIII (CD16), which is responsible for IgG-dependent cell cytotoxicity and for production of several cytokines and chemokines. Whereas Fc gamma RIII on NK cells is composed of both Fc gamma RIII alpha and FcR gamma chains, that on mast cells is distinct from NK cells
Synthetic mimics of small Mammalian cell surface receptors.
Journal:J Am Chem Soc
PubMed ID:15600339
Receptors on the surface of mammalian cells promote the uptake of cell-impermeable ligands by receptor-mediated endocytosis. To mimic this process, we synthesized small molecules designed to project anti-dinitrophenyl antibody-binding motifs from the surface of living Jurkat lymphocytes. These synthetic receptors comprise N-alkyl derivatives of 3beta-cholesterylamine as the plasma membrane anchor