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Invitrogen™
β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit
β-hydroxybutyrate (β-HB), C4H8O3, accounts for about 75% of the total ketone body. Patients with diabetic ketoacidosis have increased production of NADH, which promotes the conversion of acetoacetic acid into β-HB. Therefore, the level of β-hydroxybutyrate can be used as an index to evaluate glycosuria.
| Catalog Number | Quantity |
|---|---|
| EEA046 | 96 Tests |
Catalog number EEA046
Price (HKD)
6,949.00
Each
Quantity:
96 Tests
Price (HKD)
6,949.00
Each
With this kit, β-hydroxybutyrate dehydrogenase catalyzes the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfers electrons to WST-8 to produce a yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm. This kit is useful for researchers studying ketone body metabolism and diabetes.
Key features
- Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
- Broad range of kits across key analytes and targets within the most important metabolic pathways
- Consistent—strict quality control criteria to ensure reproducible and reliable results
For Research Use Only.
Specifications
Assay Range0.01 to 2.00 mmol/L
Assay Sensitivity0.01 mmol/L
For Use With (Equipment)Microtiter Plate Reader
Incubation Time40 min.
Interassay CV4.0%
Intraassay CV2.0%
Product TypeKit
Quantity96 Tests
Sample Volume10 μL
Shipping ConditionDry Ice, 2°C to 8°C
Target Kit Namedβ-Hydroxybutyrate (Ketone Body)
Total Assay Time60 min.
Detection MethodColorimetric
Immunoassay Kit FormatColorimetric Detection Kit
Sample TypeSerum, Plasma, Urine, Animal Tissue
Target SpeciesUniversal
Unit SizeEach
Contents & Storage
Store at 2°C to 8°C.