LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

Citations & References (14)

Invitrogen™

LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. TargetRead more

Catalog Number	Quantity
L7010	1 kit

Catalog number L7010

Price (HKD)

3,142.00

Quantity:

1 kit

Price (HKD)

3,142.00

The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC₁₈ and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell TypeMammalian Cells, Eukaryotic Cells

DescriptionLIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

Detection MethodFluorescence

Dye TypeOther Label(s) or Dye(s)

FormatTube(s), Slide(s)

Quantity1 kit

Shipping ConditionRoom Temperature

ColorGreen, Red

Emission Wavelength Range501/617

Excitation Wavelength Range484, 536 nm

For Use With (Application)Viability Assay

For Use With (Equipment)Fluorescence Microscope, Flow Cytometer

Product LineLIVE/DEAD

Product TypeCell-Mediated Cytotoxicity Kit

Unit SizeEach

Contents & Storage

Store in refrigerator at 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

Which optical filter sets are compatible with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit?

The fluorescence from both live and dead bacteria stained with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit may be viewed simultaneously with any standard fluorescein long-pass filter set. Alternatively, the fluorescent live and dead cells may be viewed separately with fluorescein and either rhodamine or Texas Red bandpass filter sets. A summary of the fluorescence microscope filter sets recommended for use with the LIVE/ DEAD Cell-Mediated Cytotoxicity Kit is shown in Table 1 on Page 2 in the manual (https://tools.thermofisher.com/content/sfs/manuals/mp07010.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (14)

Citations & References

Abstract

Evaluation of human mast cell-mediated cytotoxicity by DIOC18 target cell labeling in flow cytometry.

Authors:Ozdemir O,

Journal:J Immunol Methods

PubMed ID:17188705

'(51)Cr release assay (CRA) is still the standard method to study mast cell (MC)-mediated cytotoxicity in vitro. Non-radioactive methods e.g. MTT, Hoechst 22147 staining, have also been used. Though CRA has the benefit of being reproducible, it has several drawbacks e.g. spontaneous release and radioactivity. The basic strategy of this ... More

Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells.

Authors:Bourke S, Mason HS, Borok Z, Kim KJ, Crandall ED, Kemp PJ,

Journal:Respir Res

PubMed ID:15857506

'Lung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically, the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the ... More

Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.

Authors:Kroesen BJ, Mesander G, ter Haar JG, The TH, de Leij L

Journal:J Immunol Methods

PubMed ID:1431162

A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well ... More

Rapid flow cytometric assay for the assessment of natural killer cell activity.

Authors:Chang L, Gusewitch GA, Chritton DB, Folz JC, Lebeck LK, Nehlsen-Cannarella SL

Journal:J Immunol Methods

PubMed ID:8228287

A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably separating ... More

Human Vγ2Vδ2 T cells limit breast cancer growth by modulating cell survival-, apoptosis-related molecules and microenvironment in tumors.

Authors:

Journal:Int J Cancer

PubMed ID:23595559

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