NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
Citations & References (1)
Invitrogen™

NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)

The NuPAGE MOPS SDS Buffer Kit is designed for separation of medium- to large-size proteins on NuPAGE Bis-Tris gels andRead more
Have Questions?
Catalog NumberQuantity
NP00501 kit
Catalog number NP0050
Price (HKD)
1,103.00
Each
Add to cart
Quantity:
1 kit
Price (HKD)
1,103.00
Each
Add to cart
The NuPAGE MOPS SDS Buffer Kit is designed for separation of medium- to large-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MOPS SDS Running Buffer (20X, 500 mL, Cat. No. NP0001)
• NuPAGE Sample Reducing Agent (10X, 250 μL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Gel TypeSDS PAGE Gel
Quantity1 kit
Shipping ConditionRoom Temperature
Gel CompatibilityNuPAGE Bis-Tris Gel
Product LineNuPAGE
Product TypeRunning Buffer
Unit SizeEach
Contents & Storage
Contains
• NuPAGE™ MOPS SDS Running Buffer (20X, 500 ml)
• NuPAGE™ Sample Reducing Agent (10X, 250 μl) containing 500 mM Dithiothreitol
• NuPAGE™ Antioxidant (15 ml)
• NuPAGE™ LDS Sample Buffer (10 ml)
Store each at 4°C. Warm NuPAGE™ LDS Sample Buffer to 25°C before use.

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels) FAQs

Citations & References (1)

Citations & References
Abstract
Heat shock protein-70 expressed on the surface of cancer cells binds parathyroid hormone-related protein in vitro.
Authors:Grzesiak JJ, Smith KC, Chalberg C, Truong C, Burton DW, Deftos LJ, Bouvet M,
Journal:Endocrinology
PubMed ID:15878959
Recent studies have shown that the functions of PTH-related protein (PTHrP) and its derived peptides cannot be attributed solely to PTH/PTHrP receptor binding. The present study focused on the identification of other proteins that might bind PTHrP at the cell surface. Using affinity chromatography, we applied extracts of cell-surface biotinylated ... More