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Citations & References (4)
Invitrogen™
Streptavidin Acrylamide
Streptavidin acrylamide can be co-polymerized with acrylamide on polymeric surfaces for a uniform monolayer of immobilized streptavidin. Streptavidin acrylamide can also coat beads or microspheres of 1- to 1000-μm diameter to be utilized in a variety of affinity binding or purification applications.
| Catalog Number | Quantity |
|---|---|
| S21379 | 1 mg |
Catalog number S21379
Price (HKD)
2,529.00
Each
Quantity:
1 mg
Price (HKD)
2,529.00
Each
Streptavidin acrylamide can be co-polymerized with acrylamide on polymeric surfaces to create a uniform monolayer of immobilized streptavidin. The streptavidin can then be used for biotinylated-probe isolation, utilizing probes, enzymes, antibodies, or pharmaceutical agents. Streptavidin acrylamide can coat polymeric surfaces such as beads or microspheres that range from 1 to 1000 μm in diameter. The flexibility of using streptavidin acrylamide to coat these surfaces allows for complete customization.
Streptavidin-coated microspheres (microparticles) can be used for a variety of affinity binding applications. Streptavidin rapidly binds to biotin with a strong non-covalent bond and high affinity, allowing this amplified bound complex to remain stable within a range of temperature and pH conditions. This makes streptavidin acrylamide coated microspheres a suitable material for pharmaceutical drug delivery, 3D cell culture, bacterial isolation, and flow cytometry analysis. Streptavidin-coated beads are often utilized for DNA sequencing, PCR product cleanup, cell separation, DNA single molecule imaging studies, and in a variety of diagnostic applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeAffinity
For Use With (Application)Cell culture, bacterial isolation, flow cytometry, DNA sequencing, PCR product cleanup, cell separation, DNA single molecule imaging
Label or DyeAcrylamide
Quantity1 mg
Shipping ConditionRoom Temperature
Stationary PhaseStreptavidin
FormLiquid Suspension
TypeStreptavidin Acrylamide
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C.
Citations & References (4)
Citations & References
Abstract
Directed cell growth on protein-functionalized hydrogel surfaces.
Journal:J Neurosci Methods
PubMed ID:17368788
'Biochemical surface modification has been used to direct cell attachment and growth on a biocompatible gel surface. Acrylamide-based hydrogels were photo-polymerized in the presence of an acroyl-streptavidin monomer to create planar, functionalized surfaces capable of binding biotin-labelled proteins. Soft protein lithography (microcontact printing) of proteins was used to transfer the
Immobilization of acrylamide-modified oligonucleotides by co-polymerization.
Journal:Nucleic Acids Res
PubMed ID:9862993
A flexible chemistry for solid phase attachment of oligonucleotides is described. Oligonucleotides bearing 5'-terminal acrylamide modifications efficiently co-polymerize with acrylamide monomers to form thermally stable DNA-containing polyacrylamide co-polymers. Co-polymerization attachment is specific for the terminal acrylamide group. Stable probe-containing layers are easily fabricated on supports bearing exposed acrylic groups, including
Calcium channel and glutamate receptor activities regulate actin organization in salamander retinal neurons.
Journal:J Physiol
PubMed ID:16777935
Intracellular Ca2+ regulates a variety of neuronal functions, including neurotransmitter release, protein phosphorylation, gene expression and synaptic plasticity. In a variety of cell types, including neurons, Ca2+ is involved in actin reorganization, resulting in either actin polymerization or depolymerization. Very little, however, is known about the relationship between Ca2+ and
A streptavidin surface on planar glass substrates for the detection of biomolecular interaction.
Journal:Anal Biochem
PubMed ID:10873274
Based on the requirements of biomolecular interaction analysis on direct optical transducers, a streptavidin surface is examined. A general protocol was developed allowing the immobilization of biotinylated compounds using the rife biotin-streptavidin system. This type of surface modification can be applied to all biosensors using glass surfaces as sensor devices.