SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750
SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750
Citations & References (3)
Invitrogen™

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750

The Alexa Fluor™ 750 SAIVI™ Antibody Labeling Kit provides a convenient means to label antibodies with an optimal degree ofRead more
Have Questions?
Catalog NumberQuantity
S300461 Kit
Catalog number S30046
Price (HKD)
8,416.00
Each
Add to cart
Quantity:
1 Kit
Price (HKD)
8,416.00
Each
Add to cart
The Alexa Fluor™ 750 SAIVI™ Antibody Labeling Kit provides a convenient means to label antibodies with an optimal degree of labeling for in vivo imaging applications (DOL; ∼2) over a 6-fold antibody concentration range with no adjustments in reaction volume, dye concentration, or antibody concentration necessary. Using this procedure, optimally labeled antibodies are ready for applications that require azide-free reagents, such as live-cell imaging or direct injection into animals.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorInfrared
Detection MethodFluorescence
Excitation/Emission749/775
Label TypeAlexa Fluor Dyes
Labeling Scale0.5 to 3 mg
Product LineAlexa Fluor, SAIVI
Product TypeLabeling Kit
Quantity1 Kit
Shipping ConditionRoom Temperature
Labeling TargetAntibodies
Label or DyeAlexa Fluor 750
Unit SizeEach
Contents & Storage
Store in refrigerator 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (3)

Citations & References
Abstract
Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.
Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,
Journal:Clin Cancer Res
PubMed ID:18559604
'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More
A leukocyte-mimetic magnetic resonance imaging contrast agent homes rapidly to activated endothelium and tracks with atherosclerotic lesion macrophage content.
Authors:McAteer MA, Mankia K, Ruparelia N, Jefferson A, Nugent HB, Stork LA, Channon KM, Schneider JE, Choudhury RP,
Journal:Arterioscler Thromb Vasc Biol
PubMed ID:22499989
Endothelial cell activation is an important mediator of monocyte recruitment to sites of vascular inflammation. We hypothesized that high-affinity dual-ligand microparticles of iron oxide (MPIO), targeted to P-selectin and vascular cell adhesion molecule-1 (PV-MPIO), would identify activated endothelial cells during atherosclerosis progression. In vivo magnetic resonance imaging in apolipoprotein E-deficient ... More
Blocking ephrinB2 with highly specific antibodies inhibits angiogenesis, lymphangiogenesis, and tumor growth.
Authors:Abéngozar MA, de Frutos S, Ferreiro S, Soriano J, Perez-Martinez M, Olmeda D, Marenchino M, Cañamero M, Ortega S, Megias D, Rodriguez A, Martínez-Torrecuadrada JL,
Journal:Blood
PubMed ID:22446484
Membrane-anchored ephrinB2 and its receptor EphB4 are involved in the formation of blood and lymphatic vessels in normal and pathologic conditions. Eph/ephrin activation requires cell-cell interactions and leads to bidirectional signaling pathways in both ligand- and receptor-expressing cells. To investigate the functional consequences of blocking ephrinB2 activity, 2 highly specific ... More