SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays.

Features of SuperScript VILO Master Mix include:
• Starting material can range up to 2.5 μg of total RNA in a 20 μL cDNA synthesis reaction
• Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
• RT is engineered with reduced RNase H activity and increased thermal stability

For superior cDNA synthesis performance in RT-qPCR applications
SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.