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The pBAD-DEST49 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:
• The araBAD promoter for tightly regulated expression in E. coli
• HP-thioredoxin fusion for improved protein yield and solubility
• Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
• C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
• R sites for efficient recombination with any attL-flanked Gateway™ entry vector
• The araBAD promoter for tightly regulated expression in E. coli
• HP-thioredoxin fusion for improved protein yield and solubility
• Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
• C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
• R sites for efficient recombination with any attL-flanked Gateway™ entry vector
For Research Use Only. Not for use in diagnostic procedures.
規格
Constitutive or Inducible System
Inducible
Inducing Agent
Arabinose
Promoter
araBAD
Product Type
Gateway System Destination Expression Vector
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Protein Tag
His Tag (6x), V5 Epitope Tag, Thioredoxin
Cloning Method
Gateway
Vector
pBAD, pDEST
Product Line
Gateway™
內容物與存放
The pBAD-DEST49 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.
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