Gateway™ pYES-DEST52 Vector

引用資料與參考文獻 (10)

Invitrogen™

Gateway™ pYES-DEST52 Vector

The pYES2-DEST52 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination深入閱讀

產品號碼	Quantity
12286019	6 μg

產品號碼 12286019

價格 (HKD)

3,871.00

新增至購物車

Quantity:

價格 (HKD)

3,871.00

新增至購物車

The pYES2-DEST52 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent expression in Saccharomyces cerevisiae. As part of the YES™ Vector Collection, pYES2-DEST52 carries the promoter and enhancer sequences from the GAL1 gene for regulated expression in S.cerevisiae.
pYES2-DEST52 features:

• 2μ origin of replication for high-copy maintenance
gene for auxotrophic selection on economical minimal medium
• C-terminal V5 epitope and polyhistidine(6xHis) tags for efficient detection and purification
tR sites for efficient recombination with any attL-flanked Gateway™ entry vector

For Research Use Only. Not for use in diagnostic procedures.

Antibiotic Resistance BacterialAmpicillin (AmpR)

Product TypeGateway System Destination Expression Vector

Quantity6 μg

VectorpYES, pDEST

Cloning MethodGateway

Product LineGateway

PromoterGAL1

Protein TagHis Tag (6x), V5 Epitope Tag

Unit SizeEach

內容物與存放

The pYES2-DEST52 Gateway™ destination vector is provided supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

常見問答集 (常見問題)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pYES-DEST52 Vector FAQs

引用資料與參考文獻 (10)

引用資料與參考文獻

Abstract

Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.

Authors:Waas WF, de Crécy-Lagard V, Schimmel P,

Journal:J Biol Chem

PubMed ID:16162496

'A large number of post-transcriptional base modifications in transfer RNAs have been described (Sprinzl, M., Horn, C., Brown, M., Ioudovitch, A., and Steinberg, S. (1998) Nucleic Acids Res. 26, 148-153). These modifications enhance and expand tRNA function to increase cell viability. The intermediates and genes essential for base modifications in ... More

Participation of an Endomembrane Cation/H+ Exchanger AtCHX20 in Osmoregulation of Guard Cells.

Authors:Padmanaban S, Chanroj S, Kwak JM, Li X, Ward JM, Sze H,

Journal:Plant Physiol

PubMed ID:17337534

'Guard cell movement is induced by environmental and hormonal signals that cause changes in turgor through changes in uptake or release of solutes and water. Several transporters mediating these fluxes at the plasma membrane have been characterized, however less is known about transport at endomembranes. CHX20, a member of a ... More

Identification of a receptor-independent activator of G protein signaling (AGS8) in ischemic heart and its interaction with Gbetagamma.

Authors:Sato M, Cismowski MJ, Toyota E, Smrcka AV, Lucchesi PA, Chilian WM, Lanier SM,

Journal:Proc Natl Acad Sci U S A

PubMed ID:16407149

'As part of a broader effort to identify postreceptor signal regulators involved in specific diseases or organ adaptation, we used an expression cloning system in Saccharomyces cerevisiae to screen cDNA libraries from rat ischemic myocardium, human heart, and a prostate leiomyosarcoma for entities that activated G protein signaling in the ... More

Isolation and characterization of cDNAs encoding an enzyme with glucosyltransferase activity for cyclo-DOPA from four o'clocks and feather cockscombs.

Authors:Sasaki N, Wada K, Koda T, Kasahara K, Adachi T, Ozeki Y,

Journal:Plant Cell Physiol

PubMed ID:15695438

cDNAs encoding an enzyme with UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase activity were isolated from four o'clocks and feather cockscombs. Phylogenetic analysis of the amino acid sequences deduced from the cDNAs show that they represent a single subclade distinct from those of other phenylpropanoid and flavonoid glucosyltransferases. Changes in the amount of transcripts of ... More

Molecular and Biochemical Characterization of a Novel Intracellular Invertase from Aspergillus niger with Transfructosylating Activity.

Authors:Goosen C, Yuan XL, van Munster JM, Ram AF, van der Maarel MJ, Dijkhuizen L,

Journal:Eukaryot Cell

PubMed ID:17293485

A novel sub-family of putative intracellular invertase enzymes (glycoside hydrolase family 32) has previously been identified in fungal genomes. Here we report phylogenetic, molecular and biochemical characteristics of SucB, one of two novel intracellular invertases identified in Aspergillus niger. The sucB gene was expressed in Escherichia coli and an invertase ... More

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