DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5' phosphate. Ribonuclease has been reduced to non-detectable levels.
Applications DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating random fragments for dideoxy sequencing.
NOTE: for removing DNA from RNA preparations, use Amplification Grade DNase I (Cat. No. 18068-015).
Source DNase I is purified from bovine pancreas.
Specific activity The specific activity of DNase I is typically in the range of 10,000–25,000 units/mg.
For Research Use Only. Not for use in diagnostic procedures.
規格
Concentration
50 to 375 U/μL
Shipping Condition
Wet Ice
Enzyme
DNase
Quantity
20,000 U
Product Type
DNase I
內容物與存放
DNase I is supplied as 1 vial containing 20,000 units at a concentration of 50-375 U/μl. Store in freezer (-5°C to -30°C).