TaqMan™ β-Actin Detection Reagents
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Applied Biosystems™

TaqMan™ β-Actin Detection Reagents

Applied Biosystems™ TaqMan™ β-actin provides the necessary components for using β-actin as a normalization control in TaqMan™ real-time PCR applications深入閱讀
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產品號碼Quantity
401846100 reactions
產品號碼 401846
價格 (HKD)
3,633.00
Each
新增至購物車
Quantity:
100 reactions
價格 (HKD)
3,633.00
Each
新增至購物車
Applied Biosystems™ TaqMan™ β-actin provides the necessary components for using β-actin as a normalization control in TaqMan™ real-time PCR applications employing human nucleic acid. Either DNA or RNA can be used as a target template.

• Pre-designed primers and FAM-labeled TaqMan™ probe eliminate assay design

• Rapid assay development guidelines can minimize optimization time

• Separate components provide flexibility in assay set-up

Notes on Use

The TaqMan™ β-actin Detection Reagent kit can be used with TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).

Notice to Purchaser
1. Disclaimer of License: These products may be useful in the Polymerase Chain Reaction (“PCR”) methods covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. However, no license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.

TaqMan™ probes are covered by US Patent 5,723,591 and foreign counterparts and patents pending owned by PE Corporation (NY), and may be covered by US Patents 5,801,155 and 6,084,102 and foreign counterparts licensed to Applied Biosystems™

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
規格
Control TemplateHuman Control DNA
FormatTube
Gene SymbolACTB
Internal Probe ModificationTAMRA™ Quencher (3'), FAM (5')
No. of Reactions100 Reactions
Product LineTaqMan
Product TypeReal Time PCR Reagents
Quantity100 reactions
Shipping ConditionDry Ice
SpeciesHuman
Sufficient For100 Reactions
Targetβ-actin
Label or DyeFAM
PCR Method1-step RT-qPCR
Unit SizeEach
內容物與存放
Control Template(s)

常見問答集 (常見問題)

Can I use the TaqMan β-actin Detection Reagents (Cat. No. 401846) as an endogenous control for my gene expression studies?

The TaqMan β-actin Detection Reagents were designed to amplify genomic DNA, and thus are not recommended for use as an endogenous control for gene expression studies. However, we do offer other TaqMan assay reagents that were specifically designed for that application. Two examples are: Human ACTB (Beta Actin) Endogenous Control, Cat. No. 4310881E (TAMRA probe) or Cat. No. 4326315E (MGB probe).

There are many factors to consider when validating a specific endogenous control. For additional information on designing gene expression assays and controls, please refer to the following documents: "Protocol: TaqMan Human Endogenous Control Plate", and User Bulletin #2: "Relative Quantitation of Gene Expression". You can find copies of these documents on our website by entering the titles in the main Search field.

Can TaqMan Beta-actin Detection Reagents, Cat. No. 401846, be used for both human and mouse genomic DNA?

This particular kit was designed for human DNA. Our current Beta-actin Detection Reagents are designed for human targets only. Please check our online search tools for other Beta actin reagents for human (Hs), mouse (Mm) or rat (Rn) targets.

Where can I find the TaqMan Actin Detection Reagents (P/N 401846) manual?

Complete instructions for the TaqMan B-actin Detection Reagents can be found in the manual titled "TaqMan PCR Reagent Kit with Controls" on the product page.

引用資料與參考文獻 (1)

引用資料與參考文獻
Abstract
In situ detection of starvation-induced autophagy.
Authors:Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM,
Journal:J Histochem Cytochem
PubMed ID:16148314
Autophagy is a regulated bulk degradation process involved in many different human pathologies. Transmission electron microscopy (TEM) is currently the only reliable method for monitoring autophagy in situ. Because TEM is labor intensive, we questioned whether useful marker proteins can be found for unambiguous detection of autophagy in tissue via ... More