The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT-rich regions • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.
規格
Description
Array Install Kit, 96 x 50cm Cap, For 3730XL Genetic Analyzer
AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
Includes
1 x 800μl tube of BigDye™ Terminator v3.1 Ready Reaction Mix, 1 tube M13 (-21) Primer, 1 tube pGEM Control DNA, 2 x 1mL tubes of 5X Sequencing Buffer
No. of Reactions
100
Product Line
BigDye™ Terminator
Product Type
Sequencing Buffer
Template Compatibility
BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15Kb), PCR Amplicons
內容物與存放
• 1 × 800μl tube of BigDye™ Terminator v3.1 Ready Reaction Mix • 1 tube M13 (-21) Primer • 1 tube pGEM Control DNA • 2 × 1 ml tubes of 5X Sequencing Buffer
Store kit at -15°C to -25°C. BigDye™ sequencing buffer may be stored at 4°C.