The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT-rich regions • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.
規格
Description
Cycle Sequencing Kit, 25,000 rxns, Includes 10 x 20mL tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, 4 tubes M13 (-21) Primer, 4 tubes pGEM Control DNA, 1 x 233mL tube of 5X Sequencing Buffer (may be stored at 4°C)
Format
12-well Strips, 8-well Strip, 96- or 384-well Plate
10 x 20mL tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, 4 tubes M13 (-21) Primer, 4 tubes pGEM Control DNA, 1 x 233mL tube of 5X Sequencing Buffer (may be stored at 4°C)
No. of Reactions
25,000
Product Line
BigDye™ Terminator, BigDye™
Product Type
Sequencing Buffer
Technique
Fluorescent Dye Terminator Sequencing
Template Compatibility
BAC DNA, Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA
內容物與存放
• 10 × 20-ml tubes of BigDye™ Terminator v3.1 Ready Reaction Mix • 4 tubes M13 (-21) Primer • 4 tubes pGEM Control DNA • 1 × 233-ml tube of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.