Applied Biosystems Custom Plus TaqMan RNA assays, are the custom version of our predesigned TaqMan assays for quantitative real-time PCR. Custom Plus TaqMan RNA assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.

Features include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Custom content—easily design an assay for over 20 species
• Designed using the full bioinformatic power of the TaqMan Assay design pipeline, which uses proprietary algorithms that have generated millions of assay designs and is regarded as the benchmark in the industry for qPCR design

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Recommended master mix: TaqMan Fast Advanced Master Mix