DyLight™ 650 Maleimide

Thermo Scientific DyLight 650 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 650 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.

DyLight 650 provides vibrant far-red fluorescence with comparable or improved performance over other dyes, including Alexa Fluor™ 647 and Cy5™ dye, over a broad pH range (pH 4-9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.

Features of DyLight 650 Maleimide:

• High performance—DyLight 650 fluoresces brighter than Alexa Fluor 647 and Cy5
• Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
• Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
• Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

DyLight 650 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more about maleimide chemistry.