CaptureSelect™ Biotin Anti-IgM Conjugate consists of a 14 kDa recombinant single domain antibody fragment (V_HH affinity ligand) that specifically binds to the μ chain of human, rat, and mouse IgM antibodies. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect™ Biotin Anti-IgM Conjugate allows you to:

• Detect, quantitate, and characterize all human, rat, and mouse IgM antibodies (independent of the type of light chain), recombinant IgM monoclonal antibodies, and total IgM in human plasma and/or serum samples
• Avoid cross-binding with other isotypes such as IgG, IgA, IgD, and IgE, free light chains, or with IgM from bovine sources such as FCS

Applications for CaptureSelect™ Biotin Anti-IgM Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (V_HH) created by a proprietary technology. The V_HH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).