GeneChip™ Rat Gene 2.0 ST Array
GeneChip™ Rat Gene 2.0 ST Array
Applied Biosystems™

GeneChip™ Rat Gene 2.0 ST Array

Comprehensive designWe understand that rats are an important model organism commonly used for studying human disease. In order to keep深入閱讀
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產品號碼Number of Arrays
9021246 arrays
9025012 arrays
90212530 arrays
產品號碼 902124
價格 (HKD)
-
Number of Arrays:
6 arrays
Comprehensive design
We understand that rats are an important model organism commonly used for studying human disease. In order to keep your experiments relevant to the understanding of the transcriptome, we have designed an array based on the most recent genome content for generating genome-wide expression profiles.

The GeneChip™ Rat Gene 2.0 ST Array offers a unique feature not available in other microarrays. Unlike traditional array designs that rely on a probe(s) designed to the first exon of the gene's 3' end, the hundreds of thousands of probes in this array are designed to every exon of every transcript represented on the array.

The high transcript coverage (median of 22 probes per gene) yields accurate detection for genome-wide transcript expression changes. These arrays provide higher resolution and accuracy than many of the classical 3'-biased microarray solutions available. The whole-transcript analysis approach enables researchers to detect multiple transcript isoforms from a given gene, including those that could be missed using a 3'-biased expression design, such as splice variants, non-polyadenylated transcripts, transcripts with alternative polyadenylation sites, and truncated transcripts.

Key benefits
• Whole-transcript analysis enables the capture of transcript isoforms you may miss with 3'-biased expression designs
• Comprehensive transcriptome coverage provides the best opportunity to discover interesting biology:
   - >27,000 protein coding transcripts
   - >24,000 Entrez genes
• Measure alternative splicing events/transcript variants with probes designed to maximize coverage of exons
• Reproducible: Signal correlation coefficient ≥ 0.99

Content profile
Since the design of the GeneChip Rat Gene 1.0 ST Array, there has been a substantial increase in the structural and functional understanding of the rat genome. In order to provide the research community with a tool that can measure the differential expression of this exciting class of RNA transcripts, we designed the GeneChip Rat Gene 2.0 ST Array.
For Research Use Only. Not for use in diagnostic procedures.
規格
Product LineGeneChip
Quantity6 arrays
TypeRat Gene 2.0 ST Array
ArrayTranscriptome Profiling
FormatArray Cartridge
Number of Arrays6 arrays
SpeciesRat
Unit SizeEach

常見問答集 (常見問題)

What is contained in the tab-delimited format of the GeneChip probe sequence download file?

The tab-delimited probe sequence file contains the following information:
-Probe Set Name
-Probe X: The X coordinate of the probe sequence on the GeneChip probe array.
-Probe Y: The Y coordinate of the probe sequence on the GeneChip probe array.
-Probe Interrogation Position: The base position on the consensus/exemplar sequence where the central base of the probe aligns, which is the 13th base of a 25mer probe.
-Probe Sequence: The 25-base perfect match sequence.
-Target Strandedness: The sense/antisense orientation of the target sequence that can hybridize with the probe sequence.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the NetAffx Analysis Center?

The NetAffx Analysis Center used to contain information and files regarding array content, probe sets, and functional annotations. However, the NetAffx Analysis Center has been retired. Much of the content provided by the NetAffx Analysis Center can now be accessed on the arrays’ thermofisher.com product pages or by contacting Technical Support (techsupport@thermofisher.com).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Are pseudogene databases included in the design of expression arrays?

Pseudogene databases were not included in the design of expression arrays.

How long can I store labeled cDNA when working with expression microarrays?

Labeled material can be stored for 2 weeks at -20 degrees C.

What is an Event Score in TAC 4.0 Software?

TAC 4.0 includes two algorithms for identifying alternative splicing events: the TAC 2.0 algorithm and the new EventPointer. Algorithmic determination of alternate splicing remains a challenging problem. TAC 4.0 supports two different approaches that have different sets of strengths and weaknesses. After considerable testing, the new TAC 4.0 “'Event Score” leverages both previous TAC 2.0 event estimation score and Event Pointer p-value and sorts the most likely alternative splicing events to the top. Of course, the TAC 2.0 event score and EventPointer p-values remain individually available.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.