The Precision ID DL8 Kit for the Ion Chef System is optimized for automated library construction using the Applied Biosystems Precision ID GlobalFiler NGS STR Panel v2 (Cat. No. A33114). Leveraging the automation capabilities of the Ion Chef System, the Precision ID DL8 Kit also enables the preparation of Ion AmpliSeq libraries for current commercially available Precision ID panels (e.g., Identity Panel, Ancestry Panel, mtDNA Whole Genome Panel, and mtDNA Control Region Panel) as well as custom Ion AmpliSeq SNP panels with less than 15 minutes of hands-on time and only three liquid transfer steps.

The kit offers the following:
• 125 pg minimum DNA input for library preparation with Precision ID GlobalFiler NGS STR Panel v2
• 96-well plate-based workflow and easy-to-use cartridge format with reagent barcoding
• Sufficient material for the preparation of 32 libraries (4 Ion Chef runs) and sequence analysis with the Ion 530 Chip
• Fewer components to help save time and money by preventing unnecessary waste

Barcoded library preparation
The Precision ID DL8 Kit includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. Quantitation of Precision ID GlobalFiler NGS STR Panel v2 libraries with the Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) is recommended to ensure reproducible depth of coverage between runs. Barcoded libraries can be combined and loaded onto a single Ion 530 Chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and flexible protocol
The Precision ID Library Kit uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The resulting DNA libraries are ready for downstream template preparation for clonal amplification on the Ion Chef System and sequencing on an Ion GeneStudio S5 sequencing system.