Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. NHS esters react efficiently with primary amino groups (-NH2) in pH 7–9 buffers to form stable amide bonds. The reaction results in the release of sulfo-N-hydroxy-succinimide. When exposed to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines). Sulfo-SANPAH, supplied as a sodium salt, is useful for cell-surface protein crosslinking and possesses charged groups for water solubility to a concentration of 10 mM.
Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
Features of Sulfo-SANPAH:
•
Reactive groups:
sulfo-NHS ester and
nitrophenyl azide•
Reactive towards: amino groups and any nucleophile
• Non-cleavable
•
N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate
• Optimal photolysis occurs at 320-350 nm; minimizes damage to biomolecules by irradiation
• Water soluble; non-cleavable
Product References:Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.