The Collibri ES DNA Library Preparation Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng), starting from intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about three hours with a PCR workflow. Additionally, a PCR-free version is available for inputs starting at 100 ng DNA.

The Collibri ES DNA Library Preparation Kit:
• Includes all necessary components in a single kit* for a quick use
• Is empowered on miniaturization liquid handlers to cut on costs
• Provides in-process visual feedback for easier handling
• Is suitable for conventional automation to increase throughput

* The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules, except for the Core kit, which enables Collibri combination with desired indexed adapters.

The Collibri ES DNA Library Preparation Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. Moreover, the enzymatic shearing (ES) module of the kit generates a consistently high yield of library from any amount of input DNA (including the PCR-free version), which is enough for library quantification  before sequencing and multiple runs on Illumina instruments.

Applications
The Collibri ES DNA Library Preparation Kit is recommended for:
• Whole genome sequencing
• Resequencing
• De novo assembly