SuperScript™ IV RT-LAMP Master Mix
Invitrogen™

SuperScript™ IV RT-LAMP Master Mix

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Have Questions?
變更視圖buttonViewtableView
產品號碼No. of Reactions
A518031000 Reactions
A51801100 Reactions
A51802400 Reactions
產品號碼 A51803
價格 (HKD)
19,872.00
Each
No. of Reactions:
1000 Reactions
Request bulk or custom format
價格 (HKD)
19,872.00
Each

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions. With this convenient master mix formulation, you can perform isothermal amplification from both RNA or DNA targets in a single tube format. 

Benefits of SuperScript IV RT-LAMP Master Mix
• RNA and DNA target detection using Bst DNA polymerase for isothermal amplification in as little as five minutes
• Robust and specific cDNA synthesis using SuperScript IV Reverse Transcriptase
• Enhanced specificity and sensitivity with buffer formulation and RNaseOUT recombinant ribonuclease inhibitor
• Compatible with real-time and endpoint detection—includes SYTO 9 dye for real-time detection
• Flexible design provides several options for evaluating results, including real-time and endpoint detection methods

SuperScript IV RT-LAMP Master Mix includes an evolved Bst DNA polymerase mutant with a fast reaction speed and increased sensitivity and tolerance to inhibitors, as well as SuperScript IV Reverse Transcriptase with increased processivity, thermostability, and inhibitor resistance. To prevent RNA degradation by carry-over RNases, the master mix includes the potent, noncompetitive RNaseOUT Recombinant Ribonuclease Inhibitor. Our proprietary reaction buffer composition is optimized to increase specificity by delaying no-template control (NTC) amplification time.

規格
Concentration2X
Detection MethodReal-time and End-point Detection
Includes Label or DyeSYTO 9 Green Fluorescent Nucleic Acid Stain
No. of Reactions1000 Reactions
Optimal Reaction Temperature65°C
Reaction Time5 to 15 minutes
Reverse TranscriptaseSuperScript IV
Shipping ConditionDry Ice
For Use With (Application)LAMP, RT-LAMP
PolymeraseBst DNA Polymerase
Product TypeMaster Mix
Quantity1000 reactions, 10 x 1.25 mL
Unit SizeEach
內容物與存放

• 2X SuperScript IV RT-LAMP Master Mix (10 x 1.25 mL)
• SYTO Green Fluorescent Nucleic Acid Stain (30 μL)

Store at –20°C and protect from light.

常見問答集 (常見問題)

Is it possible to combine an (RT-)LAMP reaction with colorimetric detection methods using SuperScript IV RT-LAMP Master Mix?

Yes, LAMP can be combined with colorimetric detection using calcein. We recommend adding calcein and MnCl₂ to the reaction mixture at final concentrations of 80 µM and 0.25 mM, respectively. Prior to amplification, calcein binds to Mn²⁺ ions, giving the mixture an orange color. During amplification, Mn²⁺ ions form a complex with pyrophosphate, releasing calcein and causing the reaction mixture to change to a green or yellowish-green color, indicating successful amplification.