Recombinant Proteinase K Solution (20 mg/mL)

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Proteinase K remains active:

• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g., in the presence of SDS or urea)
• In the presence of metal chelating agents (e.g., EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications

Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing

Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition

One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.