Human Dermal Fibroblasts, adult (HDFa)
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Human Dermal Fibroblasts, adult (HDFa)

Primary Human Dermal Fibroblasts, adult (HDFa) are isolated from adult skin and cryopreserved at the end of the primary culture.The深入閱讀
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產品號碼Quantity
C0135C1 vial
產品號碼 C0135C
價格 (HKD)
-
Quantity:
1 vial
Primary Human Dermal Fibroblasts, adult (HDFa) are isolated from adult skin and cryopreserved at the end of the primary culture.

The cells are:
• Performance tested—at least 12 population doublings when cultured in Medium 106 supplemented with Low Serum Growth Supplement (LSGS)
• Guaranteed to be ≥ 70% viable
• Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 viruses

Each vial contains over 0.5 x106 viable cells that have been cryopreserved at the end of the primary culture stage in a medium containing DMSO.
For Research Use Only. Not for use in diagnostic procedures.
規格
Donor SourceSingle Donor
No. of Cells≥500000
Product LineCascade Biologics
Product TypeHuman Fibroblasts
Quantity1 vial
Tested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1
AgeAdult
Cell TypeFibroblast Cells (Dermal)
Culture TypeAdherent Cell Culture
FormCryopreserved
SpeciesHuman
Unit SizeEach
內容物與存放
Store in liquid nitrogen.

常見問答集 (常見問題)

Can I get passage information for Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C)?

Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C) are primary human dermal fibroblasts isolated from adult skin and neonatal foreskin, respectively. They are cryopreserved at the end of the primary culture stage and not passaged.

What are your Human Dermal Fibroblast (HDF) cells grown in?

HDF cells are grown in Medium 106 with the addition of Low Serum Growth Supplement.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

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