Human Epidermal Melanocytes, adult, lightly pigmented donor, (HEMa-LP)
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Human Epidermal Melanocytes, adult, lightly pigmented donor, (HEMa-LP)

Primary human epidermal melanocytes isolated from adult skin, cryopreserved at the end of the secondary culture. These cells are isolated深入閱讀
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產品號碼Quantity
C0245C1 vial
產品號碼 C0245C
價格 (HKD)
-
Quantity:
1 vial

Primary human epidermal melanocytes isolated from adult skin, cryopreserved at the end of the secondary culture. These cells are isolated from lightly pigmented tissue.

  • Viability at least 70%
  • ≥500,000 viable cells/vial
  • Capable of at least 16 population doublings

Tested for:

  • Mycoplasma: Not Detected
  • Hepatitis B: Not Detected
  • Hepatitis C: Not Detected
  • HIV-1: Not Detected
  • Bacteria, yeast, and other fungi: Not Detected
  • Positive for Mel-5 antigen in the fourth culture after thawing
For Research Use Only. Not for use in diagnostic procedures.
規格
Donor AttributesLightly Pigmented
Donor SourceSingle Donor
No. of Cells≥500,000
Product LineCascade Biologics
Product TypeHuman Epidermal Melanocyte
Quantity1 vial
Tested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria and Fungi), Mycoplasma, Growth Performance Assay, HIV-1
AgeAdult
Cell TypeMelanocyte Cells
Culture TypeAdherent Cell Culture
FormCryopreserved
SpeciesHuman
Unit SizeEach
內容物與存放
Liquid nitrogen temperature

常見問答集 (常見問題)

Do you have ethnicity information for your human epidermal melanocyte donor lines?

We do provide age/sex/pigmentation/ethnicity on the Certificate of Analysis (COA).

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

Are primary cells isolated from tissue using enzymatic digestion?

Yes, our primary cells are isolated from tissue using enzymatic digestion.

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