Search Thermo Fisher Scientific
產品概覽
圖表
影片
建議
建議
文件
常見問題
引用資料與參考文獻
其他資訊
建議
Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng
• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance
The ChargeSwitch™ Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch™ purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance
The ChargeSwitch™ Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch™ purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
For Research Use Only. Not for use in diagnostic procedures.
規格
Format
Kit
Isolation Technology
ChargeSwitch Chemistry
Sample Type
Yeast
Scale
Mini
Final Product Type
Plasmid DNA
For Use With (Application)
PCR, Cloning, Southern Blotting, Sequencing, Transformation
Green Features
Less hazardous, Fewer resources used and less waste
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
50 Preps
Prep Scale
< 100 μg (Small-Scale) Plasmid DNA
Product Line
ChargeSwitch™
Product Type
Plasmid Yeast Mini Kit
Quantity
50 Preps
Shipping Condition
Room Temperature
Target
Plasmid DNA
內容物與存放
All components of the ChargeSwitch™ Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store Precipitation Buffer (N5) at 4°C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.
Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Resuspension Buffer (R4)
• ChargeSwitch™ Lysis Buffer (L9)
• ChargeSwitch™ Precipitation Buffer (N5)
• ChargeSwitch™ Wash Buffer (W11)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
圖表
文件與下載
證書
依批號或部分批號搜尋
常見問答集 (常見問題)
引用資料與參考文獻
Search citations by name, author, journal title or abstract text