Pyrophosphatase, inorganic (0.1 U/μL)

Thermo Scientific Pyrophosphatase, Inorganic catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates. The enzyme requires a divalent metal cation with Mg²⁺ conferring the highest activity.

Highlights

• Active in Thermo Scientific buffers for DNA polymerases, RNA polymerases, and Reverse Transcriptases

Applications

• High yield RNA synthesis by in vitro transcription (see Reference 2)
• DNA polymerization reactions: preventing accumulation of pyrophosphate (see References 3, 4)
• Removal of contaminant PP_i in reagents used for SNP genotyping by methods based on the detection of pyrophosphate

Note

The enzyme can be diluted with the supplied storage (dilution) buffer. Use of this enzyme in certain applications may be covered by patents and may require a license.