Palladium API Fluorescent Detection Kit

The Palladium Detection research-use-only kit is a fluorescent assay designed for the quantification and detection of palladium in various solvents including toluene, ethanol, acetonitrile, DMSO and DMF containing samples.

This complete, ready-to-use kit includes black 96-well plate(s), palladium standard, palladium detection reagent, and other components to perform the assay. A 96-well microplate reader capable of reading fluorescent emission at 520 nm, with excitation at 485 nm, is required for use of this kit.

Performance characteristics
• Assay type: fluorescent detection kit
• Sample types: various solvents including toluene, ethanol, acetonitrile, DMSO, and DMF
• Standard curve range: 3.1–100 nM
• Reactivity: species independent

Background
Many new synthetic transformations that have been developed in recent years use palladium (Pd) compounds for the catalysis of carbon-carbon and carbon-heteroatom coupling reactions such as the Buchwald-Hartwig, Heck, Kumada, Negishi, Nozaki-Hiyama, Sonogashira, Stille, Suzuki-Miyaura, and Tsuji-Trost transformations. These reactions have found increased popularity for pharmaceutical processes as they utilize a wide-range of functional groups and can therefore be used to build complicated molecules. However, palladium-catalyzed reactions present a problem in that the palladium can often be retained in the isolated product.

Assay principle
The standard methods of quantifying palladium in samples are atomic absorption analysis, x-ray fluorescence, and plasma emission spectroscopy such as inductively-coupled plasma mass spectroscopy (ICP-MS). These methods require expensive instruments with a highly trained scientist to operate. Cross-contamination of the instrument can limit the throughput of this analysis and requires scrupulous clean up methodology.

This Palladium Fluorescent Detection Kit is designed to allow the rapid determination of the relative amounts of Pd present in a variety of samples and solvents including toluene, ethanol, acetonitrile, DMSO, and DMF. The kit uses a non-fluorescent detection molecule that, in the presence of palladium, cleaves to yield a brightly fluorescent product.

Related links
Learn more about ELISA kits
Learn more about other immunoassays