T4 DNA Polymerase (5 U/μL)

Thermo Scientific T4 DNA Polymerase is a template-dependent DNA polymerase that catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.

Highlights

• Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I (Cat. No. EP0041), E. coli,and Klenow fragment (Cat. No. EP0051)
• Active in Thermo Scientific restriction enzyme, PCR, RT and T4 DNA Ligase (Cat. No. EL0011) buffers

Applications

• Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs(see References1, 2)
• Blunting of PCR products with 3'-dA overhangs
• Synthesis of labeled DNA probes by the replacement reaction(see Reference3)
• Oligonucleotide-directed site-specific mutagenesis(see Reference4)
• Ligation-independent cloning of PCR products