Fluorescein Di-β-D-Glucopyranoside (FDGlu)

引用資料與參考文獻 (5)

Invitrogen™

Fluorescein Di-β-D-Glucopyranoside (FDGlu)

Cell permeant

產品號碼	Quantity
F2881 亦稱為 F-2881	5 mg

產品號碼 F2881

亦稱為 F-2881

價格 (HKD)

2,147.00

Quantity:

價格 (HKD)

2,147.00

Fluorescein di-β-D-glucopyranoside is one of the most sensitive substrates for glucosidases. The nonfluorescent substrate is sequentially hydrolyzed by β-glucosidase, first to fluorescein monoglucoside and then to highly fluorescent fluorescein. Enzyme-mediated hydrolysis of fluorescein di-β-D-glucopyranoside can be followed by the increase in either absorbance or fluorescence.

For Research Use Only. Not for use in diagnostic procedures.

Cell PermeabilityCell-permeant

Excitation/Emission490/514

For Use With (Application)Glucosidases Substrate

Quantity5 mg

Shipping ConditionRoom Temperature

SubstrateBeta-Glucosidase Substrate

Detection MethodFluorescence

Emission Wavelength Range517 nm

Excitation Wavelength Range490 nm

Substrate PropertiesChemical Substrate

Unit SizeEach

內容物與存放

Store in freezer (-5 to -30°C).

引用資料與參考文獻 (5)

引用資料與參考文獻

Abstract

An in situ study of beta-glucosidase activity in normal and Gaucher fibroblasts with fluorogenic probes.

Authors:Kohen E, Kohen C, Hirschberg JG, Santus R, Grabowski G, Mangel W, Gatt S, Prince J

Journal:Cell Biochem Funct

PubMed ID:8403230

'Beta-glucosidase activity was evaluated in situ by means of fluorogenic probes in normal human fibroblasts and fibroblasts from homozygous carriers of the Gaucher trait. Probe internalization, targeting to lysosomes and post-cleavage probe retention were the primary concerns. Internalization and targeting were attempted by in situ photosensitized labilization of lysosomal membranes, ... More

Yeast exo-beta-glucanases can be used as efficient and readily detectable reporter genes in Saccharomyces cerevisiae.

Authors:Cid VJ, Alvarez AM, Santos AI, Nombela C, Sanchez M

Journal:Yeast

PubMed ID:7975893

'Yeast exo-1,3-beta-glucanases are secretable proteins whose function is basically trophic and may also be involved in cell wall glucan hydrolytic processes. Since fluorescein di(beta-D-glucopyranoside) is a fluorogenic substrate detectable and quantifiable by flow cytometry, it was used for testing the ability of the EXG1 gene product of Saccharomyces cerevisiae and ... More

A flow cytometric assay for lysosomal glucocerebrosidase.

Authors:van Es HH, Veldwijk M, Havenga M, Valerio D

Journal:Anal Biochem

PubMed ID:9177687

'A flow cytometric assay is described for the determination of glucocerebrosidase (GC) activity using fluorescein di-beta-glucopyranoside (FDGlu). Fluorescent product is formed upon intracellular hydrolysis of FDGlu and is measured in the FL1 channel of a flow cytometer. We show that the assay is specific for lysosomal beta-glucosidase or glucocerebrosidase (1) ... More

Directed evolution of a thermophilic beta-glucosidase for cellulosic bioethanol production.

Authors:Hardiman E, Gibbs M, Reeves R, Bergquist P,

Journal:Appl Biochem Biotechnol

PubMed ID:19834652

Characteristics that would make enzymes more desirable for industrial applications can be improved using directed evolution. We developed a directed evolution technique called random drift mutagenesis (RNDM). Mutant populations are screened and all functional mutants are collected and put forward into the next round of mutagenesis and screening. The goal ... More

Nucleic acid detection using non-radioactive labelling methods.

Authors:Mansfield ES, Worley JM, McKenzie SE, Surrey S, Rappaport E, Fortina P

Journal:Mol Cell Probes

PubMed ID:7477006

Nucleic acid probe-based assays are now widely used in genetic research, human identification, forensics and in a broad spectrum of clinical assays in the fields of microbiology, haematology/oncology and virology. Labelled probes are used in a variety of assay formats including dot-blots, Southern blots (DNA target), Northern blots (RNA target), ... More