FluoSpheres™ Biotin-Labeled Microspheres, 0.04 μm, yellow-green fluorescent (505/515), 1% solids

引用資料與參考文獻 (4)

Invitrogen™

FluoSpheres™ Biotin-Labeled Microspheres, 0.04 μm, yellow-green fluorescent (505/515), 1% solids

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from深入閱讀

產品號碼	Quantity
F8766 亦稱為 F-8766	0.4 mL

產品號碼 F8766

亦稱為 F-8766

價格 (HKD)

5,018.00

新增至購物車

Quantity:

0.4 mL

價格 (HKD)

5,018.00

新增至購物車

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ FluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres™ Microsphere Specifications

• Label (Ex/Em): Yellow-green fluorescent (505 / 515)
• Nominal bead diameter: 0.04 μm
• Surface modification: Biotin
• Solids: 1%

Characteristics of the Various FluoSpheres™ Coupling Surfaces
• Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres™ beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres™ beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres™ beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres™ Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 μm, 0.04 μm, 0.1 μm, 0.2 μm, 0.5 μm, 1.0 μm, 2.0 μm, 4.0 μm, 10.0 μm, and 15.0 μm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres™ beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Product LineFLUOSPHERES

Quantity0.4 mL

Shipping ConditionRoom Temperature

Surface ModificationOther Modification or Label

ColorYellow-Green

Diameter (Metric)0.04 μm

MaterialPolystyrene

Product TypeMicrosphere

Unit SizeEach

內容物與存放

Store in refrigerator (2–8°C) and protect from light.

常見問答集 (常見問題)

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

I accidentally froze my microspheres; can I still use them?

Even brief freezing can cause irreversible aggregation and potential distortion of the bead shape. You should not use these microspheres.

Find additional tips, troubleshooting help, and resources within our Microspheres Support Center.

My protein-coated microspheres appear to be non-specifically binding in my experimental system. Do you have a product that can help reduce these non-specific interactions?

Non-specific binding can often be relieved by a blocking solution, but microspheres seem to require a stronger blocking solution than those most commonly commercially available. Hence, we've developed the BlockAid Blocking Solution (Cat. No. B10710). This reagent is a protein-based blocking solution designed for use with FluoSpheres microspheres and TransFluoSpheres microspheres conjugated to biotin, streptavidin, NeutrAvidin biotin-binding protein, or other proteins. The BlockAid Blocking Solution has proven useful for reducing the nonspecific binding of protein-coated or other macromolecule-coated microspheres in a wide variety of flow cytometry, microscopy, and microarray applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Biotin-Labeled Microspheres, 0.04 μm, yellow-green fluorescent (505/515), 1% solids FAQs

引用資料與參考文獻 (4)

引用資料與參考文獻

Abstract

Nanoparticles as fluorescence labels: is size all that matters?

Authors:Swift JL, Cramb DT,

Journal:Biophys J

PubMed ID:18390610

'Fluorescent labels are often used in bioassays as a means to detect and characterize ligand-receptor binding. This is due in part to the inherently high sensitivity of fluorescence-based technology and the relative accessibility of the technique. There is often little concern raised as to whether or not the fluorescent label ... More

Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures.

Authors:Korlach J, Marks PJ, Cicero RL, Gray JJ, Murphy DL, Roitman DB, Pham TT, Otto GA, Foquet M, Turner SW,

Journal:Proc Natl Acad Sci U S A

PubMed ID:18216253

Optical nanostructures have enabled the creation of subdiffraction detection volumes for single-molecule fluorescence microscopy. Their applicability is extended by the ability to place molecules in the confined observation volume without interfering with their biological function. Here, we demonstrate that processive DNA synthesis thousands of bases in length was carried out ... More

A contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia.

Authors:Abodeely M, DuBois KN, Hehl A, Stefanic S, Sajid M, DeSouza W, Attias M, Engel JC, Hsieh I, Fetter RD, McKerrow JH,

Journal:Eukaryot Cell

PubMed ID:19749174

The dynamic evolution of organelle compartmentalization in eukaryotes and how strictly compartmentalization is maintained are matters of ongoing debate. While the endoplasmic reticulum (ER) is classically envisioned as the site of protein cotranslational translocation, it has recently been proposed to have pluripotent functions. Using transfected reporter constructs, organelle-specific markers, and ... More

Different domains of the AMPA receptor direct stargazin-mediated trafficking and stargazin-mediated modulation of kinetics.

Authors:Bedoukian MA, Weeks AM, Partin KM

Journal:J Biol Chem

PubMed ID:16793768

Stargazin is an accessory protein of AMPA receptors that enhances surface expression and also affects the biophysical properties of the receptor. AMPA receptor domains necessary for either of these two processes have not yet been identified. Here, we used confocal imaging and electrophysiology of heterologously expressed, fluorophore-tagged GluR1, GluR2, and ... More