FluoSpheres™ Carboxylate-Modified Microspheres
FluoSpheres™ Carboxylate-Modified Microspheres
Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.
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產品號碼Diameter (Metric)ColorExcitation/EmissionQuantity
F88010.1 μmRed580/605 nm10 mL
F88030.1 μmYellow-Green505/515 nm10 mL
F88161.0 μmCrimson625/645 nm2 mL
F88231.0 μmYellow-Green505/515 nm10 mL
F88110.2 μmYellow-Green505/515 nm10 mL
F88070.2 μmDark Red660/680 nm2 mL
F107200.04 μmYellow-Green, Orange, Red, Dark Red505/515, 540/560, 580/605, 660/680 nm1 mL/each
F208810.2 μmOrange365/610 nm2 mL
F8783
亦稱為 F-8783
0.02 μmDark Red660/680 nm2 mL
F87860.02 μmRed580/605 nm10 mL
F8795
亦稱為 F-8795
0.04 μmYellow-Green505/515 nm1 mL
F88130.5 μmYellow-Green505/515 nm10 mL
F88201.0 μmOrange540/560 nm10 mL
F88252.0 μmNile Red535/575 nm2 mL
F88272.0 μmYellow-Green505/515 nm2 mL
F8781
亦稱為 F-8781
0.02 μmBlue365/415 nm10 mL
F8782
亦稱為 F-8782
0.02 μmCrimson625/645 nm2 mL
F87840.02 μmNile Red535/575 nm10 mL
F87870.02 μmYellow-Green505/515 nm10 mL
F8789
亦稱為 F-8789
0.04 μmDark Red660/680 nm1 mL
F8792
亦稱為 F-8792
0.04 μmOrange540/560 nm1 mL
F87930.04 μmRed580/605 nm1 mL
F87940.04 μmRed-Orange565/580 nm1 mL
F87970.1 μmBlue350/440 nm10 mL
F87990.1 μmInfrared715/755 nm1 mL
F8800
亦稱為 F-8800
0.1 μmOrange540/560 nm10 mL
F88050.2 μmBlue365/415 nm10 mL
F88060.2 μmCrimson625/645 nm2 mL
F88090.2 μmOrange540/560 nm10 mL
F88100.2 μmRed580/605 nm10 mL
F88120.5 μmRed580/605 nm10 mL
F88141.0 μmBlue365/415 nm10 mL
F88151.0 μmBlue350/440 nm10 mL
F88191.0 μmNile Red535/575 nm10 mL
F88211.0 μmRed580/605 nm10 mL
F8824
亦稱為 F-8824
2.0 μmBlue365/415 nm2 mL
F88262.0 μmRed580/605 nm2 mL
產品號碼 F8801
價格 (HKD)
4,041.00
Each
新增至購物車
Diameter (Metric):
0.1 μm
Color:
Red
Excitation/Emission:
580/605 nm
Quantity:
10 mL
價格 (HKD)
4,041.00
Each
新增至購物車

Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.

Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.
For Research Use Only. Not for use in diagnostic procedures.
規格
Excitation/Emission580/605 nm
Product LineFLUOSPHERES
Quantity10 mL
Surface ModificationCarboxylate
ColorRed
Diameter (Metric)0.1 μm
For Use With (Application)Fluorescence Microscopy
MaterialPolystyrene
Product TypeCarboxylate-Modified Microsphere
Unit SizeEach
內容物與存放
Store in refrigerator (2°C to 8°C) and protect from light.

常見問答集 (常見問題)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

FluoSpheres Carboxylate-Modified Microspheres (Cat. Nos. F8783, F8786, F8801, F8807, F8811, F8803, F8816, F8823, F20881, F10720) have a 1-year warranty guarantee, unless otherwise indicated on the Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用資料與參考文獻 (14)

引用資料與參考文獻
Abstract
Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.
Authors:Gustafsson MG, Shao L, Carlton PM, Wang CJ, Golubovskaya IN, Cande WZ, Agard DA, Sedat JW,
Journal:Biophys J
PubMed ID:18326650
'Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resolution, with true ... More
Mapping mechanical strain of an endogenous cytoskeletal network in living endothelial cells.
Authors:Helmke BP, Rosen AB, Davies PF
Journal:Biophys J
PubMed ID:12668477
'A central aspect of cellular mechanochemical signaling is a change of cytoskeletal tension upon the imposition of exogenous forces. Here we report measurements of the spatiotemporal distribution of mechanical strain in the intermediate filament cytoskeleton of endothelial cells computed from the relative displacement of endogenous green fluorescent protein (GFP)-vimentin before ... More
a2 Adrenergic receptor-mediated inhibition of thermogenesis.
Authors:Madden CJ, Tupone D, Cano G, Morrison SF,
Journal:J Neurosci
PubMed ID:23365239
'a2 adrenergic receptor (a2-AR) agonists have been used as antihypertensive agents, in the management of drug withdrawal, and as sedative analgesics. Since a2-AR agonists also influence the regulation of body temperature, we explored their potential as antipyretic agents. This study delineates the central neural substrate for the inhibition of rat ... More
Glucoprivation in the ventrolateral medulla decreases brown adipose tissue sympathetic nerve activity by decreasing the activity of neurons in raphe pallidus.
Authors:Madden CJ,
Journal:Am J Physiol Regul Integr Comp Physiol
PubMed ID:22071154
'In urethane/a-chloralose anesthetized rats, cold exposure increased brown adipose tissue sympathetic nerve activity (BAT SNA: +699 ± 104% control). Intravenous administration of 2-deoxy-D-glucose (2-DG; 200 mg·ml(-1)·kg(-1)) reversed the cold-evoked activation of BAT SNA (nadir: 139 ± 36% of control) and decreased BAT temperature (-1.1 ± 0.2°C), expired CO(2) (-0.4 ± ... More
Spatiotemporal analysis of flow-induced intermediate filament displacement in living endothelial cells.
Authors:Helmke BP, Thakker DB, Goldman RD, Davies PF
Journal:Biophys J
PubMed ID:11159394
'The distribution of hemodynamic shear stress throughout the arterial tree is transduced by the endothelium into local cellular responses that regulate vasoactivity, vessel wall remodeling, and atherogenesis. Although the exact mechanisms of mechanotransduction remain unknown, the endothelial cytoskeleton has been implicated in transmitting extracellular force to cytoplasmic sites of signal ... More