Mag-fura-2, tetrapotassium salt is an intracellular magnesium indicator that is ratiometric and UV light-excitable. This water-soluble salt form is useful深入閱讀
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產品號碼
Quantity
M1290
1 mg
產品號碼 M1290
價格 (HKD)
4,149.00
Each
新增至購物車
Quantity:
1 mg
價格 (HKD)
4,149.00
Each
新增至購物車
Mag-fura-2, tetrapotassium salt is an intracellular magnesium indicator that is ratiometric and UV light-excitable. This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I14402).
For Research Use Only. Not for use in diagnostic procedures.
規格
Detection MethodFluorescence
Dye TypeFluorescent Dye-Based
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Equipment)Fluorescence Microscope, Microphotometer, Flow Cytometer
Product TypeMagnesium Indicator
Unit SizeEach
內容物與存放
Store in freezer -5°C to -30°C and protect from light.
引用資料與參考文獻 (86)
引用資料與參考文獻
Abstract
Ca2+ transients in cardiac myocytes measured with high and low affinity Ca2+ indicators.
Authors:Berlin JR,Konishi M
Journal:Biophysical journal
PubMed ID:8274651
Intracellular calcium ion ([Ca2+]i) transients were measured in voltage-clamped rat cardiac myocytes with fura-2 or furaptra to quantitate rapid changes in [Ca2+]i. Patch electrode solutions contained the K+ salt of fura-2 (50 microM) or furaptra (300 microM). With identical experimental conditions, peak amplitude of stimulated [Ca2+]i transients in furaptra-loaded myocytes ... More
Matrix free Mg2+ changes with metabolic state in isolated heart mitochondria.
Authors:Jung DW, Apel L, Brierley GP
Journal:Biochemistry
PubMed ID:2361136
'The concentration of free Mg2+ in the matrix of isolated heart mitochondria has been monitored by using the fluorescent probe furaptra (mag-fura-2). Beef heart mitochondria respiring in a KCl medium in the absence of external Mg2+ maintain free matrix Mg2+ near 0.50 mM. Addition of Pi under these conditions decreases ... More
Modulation of Mg2+ efflux from rat ventricular myocytes studied with the fluorescent indicator furaptra.
Authors:Tursun P, Tashiro M, Konishi M
Journal:Biophys J
PubMed ID:15626700
'The fluorescent Mg(2+) indicator furaptra (mag-fura-2) was introduced into single ventricular myocytes by incubation with its acetoxy-methyl ester form. The ratio of furaptra's fluorescence intensity at 382 and 350 nm was used to estimate the apparent cytoplasmic [Mg(2+)] ([Mg(2+)](i)). In Ca(2+)-free extracellular conditions (0.1 mM EGTA) at 25 degrees C, ... More
Mg2+ buffering in cultured chick ventricular myocytes: quantitation and modulation by Ca2+.
Authors:Koss KL, Putnam RW, Grubbs RD
Journal:Am J Physiol
PubMed ID:8498485
'To characterize the Mg2+ buffering of cultured chick ventricular myocytes, cytosolic Mg2+ was increased by liberating Mg2+ normally chelated by ATP upon total depletion of ATP content. Because the total Mg content and cell volume remained constant during this time, the difference between the amount of Mg2+ liberated (2.7 mM) ... More
Capacitative Ca2+ entry is closely linked to the filling state of internal Ca2+ stores: a study using simultaneous measurements of ICRAC and intraluminal [Ca2+].
Authors:Hofer AM, Fasolato C, Pozzan T
Journal:J Cell Biol
PubMed ID:9442108
'ICRAC (the best characterized Ca2+ current activated by store depletion) was monitored concurrently for the first time with [Ca2+] changes in internal stores. To establish the quantitative and kinetic relationship between these two parameters, we have developed a novel means to clamp [Ca2+] within stores of intact cells at any ... More