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Gibco™

Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Male

Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Male have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.
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產品號碼Quantity
MSCP101.5 mL
產品號碼 MSCP10
價格 (HKD)
15,165.00
Each
Quantity:
1.5 mL
價格 (HKD)
15,165.00
Each

These plateable cryopreserved male mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO™ Hepatocytes

High quality hepatocytes, with high viabilities, proper cell morphology, and metabolic function increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

  • Viability ≥75%
  • Attachment efficiency
  • Monolayer formation and integrity
  • Confluency >80% on Day 3
  • Phase I and II enzyme activities

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Note:

These products require shipment in LN2 vapor dewars therefore additional fees may apply.

For Research Use Only. Not for use in diagnostic procedures.
規格
Donor SourcePooled
No. of Cells4-8 x 106
Product LineGibco
Product TypeHepatocytes
Quantity1.5 mL
Cell LinePrimary
Cell TypeHepatocytes
Culture TypeHepatocyte Cell Culture
FormCryopreserved
GenderMale
SpeciesMouse
TissueLiver
Unit SizeEach
內容物與存放
Stored in a cryostorage dewar or -135°C freezer.

常見問答集 (常見問題)

Who can I call if I have technical questions regarding cryopreserved hepatocytes?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocytes. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952 3559

With my hepatocytes, I'm seeing rounding up of the cells, cellular debris, and/or holes in the monolayer, indicating dying cells. What should I do?

This could be due to the toxicity of the test compound. Here are other potential causes and recommendations:

-Sub-optimal culture medium: Use Williams Medium E with Plating and Incubation Supplement Packs; refer to our plating protocol
-Hepatocyte lot not characterized as plateable: Check lot specifications to ensure it is qualified for plating
-Cells were cultured for too long: In general, plateable cryopreserved hepatocytes should not be cultured for more than five days

I'm getting unexpected induction results with my hepatocytes. What could be causing this?

First of all, we recommend comparing results to those reported on our lot-specific characterization specification sheet (human cells) and also referring to our enzyme induction protocol. Here are other potential causes and recommendations:

-Sub-optimal monolayer confluency: Please see our recommendations for 'I have a sub-optimal monolayer confluency for my hepatocytes. What should I do?'
-Poor monolayer integrity: Please see our recommendations for 'With my hepatocytes, I’m seeing rounding up of the cells, cellular debris, and/or holes in the monolayer, indicating dying cells. What should I do?'
-Inappropriate positive control: Check positive control to ensure suitability
-Incorrect concentration of positive control: Use the correct concentration of positive control

I'm seeing sub-optimal bile canlicular formation with my hepatocytes. What should I do?

Please see the following causes and recommendations:

-Hepatocyte lot not transporter-qualified: Check lot specifications to ensure it is transporter-qualified
-Sub-optimal culture medium: Use Williams Medium E with Plating and Incubation Supplement Packs; refer to our plating protocol
-Not enough time for bile canaliculi to form: In general, at least 4-5 days in culture is required for bile canalicular network formation

I'm getting a loss of membrane integrity or cuboidal cell shape with my hepatocytes. What should I do?

Please see the following causes and recommendations:

-Hepatocyte lot not characterized as plateable: Check lot specifications to ensure it is qualified for plating
-Sub-optimal culture medium: Use Williams Medium E with Plating and Incubation Supplement Packs; refer to our plating protocol
-Cells were cultured for too long: In general, plateable cryopreserved hepatocytes should not be cultured for more than five days

View all Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Male FAQs