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引用資料與參考文獻 (9)
Invitrogen™
pHrodo™ Green STP Ester
The amine-reactive pH-sensitive pHrodo™ Green STP ester dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis.深入閱讀
| 產品號碼 | Quantity |
|---|---|
| P35369 | 500 μg |
產品號碼 P35369
價格 (HKD)
-
Quantity:
500 μg
The amine-reactive pH-sensitive pHrodo™ Green STP ester dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic.
• Use pH-sensitive pHrodo™ Green STP ester to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others
The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms, including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.
The pHrodo™ Green STP ester is an amine reactive dye that can be used to make pHrodo™ Green bioconjugates in aqueous buffer. The STP ester will react with primary amines on a protein, cell, or virus to create a stable conjugate that can be used in live cell assays or stored for later use.
Amine reactive pHrodo™ dye is also available with red fluorescence (see pHrodo™ Red SE). In addition, thiol-reactive pHrodo™ Red Maleimide and Green Maleimide are a great alternative to amine-reactive dyes for antibody labeling. Thiol labeling ensures that the dye will not attach to the binding region of the antibody. pHrodo™ Green and Red conjugates, for example dextran, E. coli and S. aureus, are also available in ready-to-use form.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
• Use pH-sensitive pHrodo™ Green STP ester to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others
The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms, including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.
The pHrodo™ Green STP ester is an amine reactive dye that can be used to make pHrodo™ Green bioconjugates in aqueous buffer. The STP ester will react with primary amines on a protein, cell, or virus to create a stable conjugate that can be used in live cell assays or stored for later use.
Amine reactive pHrodo™ dye is also available with red fluorescence (see pHrodo™ Red SE). In addition, thiol-reactive pHrodo™ Red Maleimide and Green Maleimide are a great alternative to amine-reactive dyes for antibody labeling. Thiol labeling ensures that the dye will not attach to the binding region of the antibody. pHrodo™ Green and Red conjugates, for example dextran, E. coli and S. aureus, are also available in ready-to-use form.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
規格
Chemical ReactivityAmine
Detection MethodFluorescence
EC Extinction Coefficient75000
Emission525
Excitation505
Label or DyepHrodo™ Green
Product TypeSTP Ester
Quantity500 μg
Reactive GroupSTP Ester
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
ColorGreen
Product LinepHrodo
Unit SizeEach
內容物與存放
1 vial , store ≤ -20 °C, dessicate and protected from light.
常見問答集 (常見問題)
I am performing a phagocytosis assay of macrophages engulfing pHrodo-labeled bacteria. What do you recommend for fixation after the phagocytosis?
引用資料與參考文獻 (9)
引用資料與參考文獻
Abstract
The well-coordinated linkage between acidogenicity and aciduricity via insoluble glucans on the surface of Streptococcus mutans.
Journal:
PubMed ID:26657939
Streptococcus mutans is considered the principal cariogenic bacterium for dental caries. Despite the recognition of their importance for cariogenesis, the possible coordination among S. mutans' main virulence factors, including glucan production, acidogenicity and aciduricity, has been less well studied. In the present study, using S. mutans strains with surface-displayed pH-sensitive
Preclinical evaluation of a GFRA1 targeted antibody-drug conjugate in breast cancer.
Journal:Oncotarget
PubMed ID:29796165
'Despite recent advances in treatment, breast cancer remains the second-most common cause of cancer death among American women. A greater understanding of the molecular characteristics of breast tumors could ultimately lead to improved tumor-targeted treatment options, particularly for subsets of breast cancer patients with unmet needs. Using an unbiased genomics
Genetically Engineered iPSC-Derived FTDP-17 MAPT Neurons Display Mutation-Specific Neurodegenerative and Neurodevelopmental Phenotypes.
Journal:Stem Cell Reports
PubMed ID:30057263
'Tauopathies such as frontotemporal dementia (FTD) remain incurable to date, partially due to the lack of translational in vitro disease models. The MAPT gene, encoding the microtubule-associated protein tau, has been shown to play an important role in FTD pathogenesis. Therefore, we used zinc finger nucleases to introduce two MAPT mutations
Dietary obesity reversibly induces synaptic stripping by microglia and impairs hippocampal plasticity.
Journal:Brain Behav Immun
PubMed ID:26336035
'Obesity increases risk of age-related cognitive decline and is accompanied by peripheral inflammation. Studies in rodent models of obesity have demonstrated that impaired hippocampal function correlates with microglial activation, but the possibility that neuron/microglia interactions might be perturbed in obesity has never been directly examined. The goal of this study
Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens.
Journal:Nat Genet
PubMed ID:30397336
Phagocytosis is required for a broad range of physiological functions, from pathogen defense to tissue homeostasis, but the mechanisms required for phagocytosis of diverse substrates remain incompletely understood. Here, we developed a rapid magnet-based phenotypic screening strategy, and performed eight genome-wide CRISPR screens in human cells to identify genes regulating