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Determine of the ability of microorganisms, primarily Enterobacteriaceae, to split indole from the tryptophan molecule with Thermo Scientific™ Remel™ BactiDrop™ Kovac's Indole reagent. Earlier tests to confirm indole production required a xylene extraction procedure to detect small quantities of indole produced from the breakdown of tryptophan. Kovac’s utilized amyl alcohol instead of ethyl alcohol to bypass the extraction procedure involved in the Ehrlich-Beohme reagent1. Gadebusch and Gabriel reported that substitution of isoamyl alcohol resulted in greater stability of Kovac’s reagent2.
| Description | BactiDrop Indole, Kovacs |
| CE Marker | Yes |
| Detectable Analytes | Nitrite, Free Nitrogen Gas |
| Quantity | 50 x 0.75 mL |
| Unit Size | Each |
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Tryptophanases are intracellular enzymes that mediate indole production by hydrolysing the amino acid tryptophan. Indole combines with dimethyl-aminobenzaldehyde to form a red compound. This is a condensation product formed by an acid splitting of a protein3.Ready-to-use: Liquid reagentEasy to interpret: Positive Test - A pink-red ring between the broth and reagentNegative Test - No color developmentKovac's Indole Reagent contains 5% w/v ρ-dimethylaminobenzaldehyde (CAS 100-10-7), hydrochloric acid (concentrated) (CAS 7647-01-0) and amyl alcohol (CAS 71-41-0) and is supplied as 0.75 mL ampules..Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
- Kovacs, N.Z. 1928. Immunforsch. 55:311.
- Gadebusch, H.H. and S. Gabriel. 1956. Am. J. Clin. Pathol. 26:1373-1375.
- MacFaddin, J.F. 2000. Biochemical Tests for Identification of Medical Bacteria. 3rd Lippincott Williams & Wilkins, Philadelphia, P.A.
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