Streptavidin, Alexa Fluor™ 430 conjugate
Streptavidin, Alexa Fluor™ 430 conjugate
Invitrogen™

Streptavidin, Alexa Fluor™ 430 conjugate

Our Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ∼434/539 nm). Streptavidin, a nonglycosylated biotin-binding protein深入閱讀
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產品號碼Quantity
S11237
亦稱為 S-11237
1 mg
產品號碼 S11237
亦稱為 S-11237
價格 (HKD)
3,096.00
Each
新增至購物車
Quantity:
1 mg
價格 (HKD)
3,096.00
Each
新增至購物車
Our Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ∼434/539 nm). Streptavidin, a nonglycosylated biotin-binding protein with a near-neutral isoelectric point, reportedly exhibits less nonspecific binding than avidin.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.
For Research Use Only. Not for use in diagnostic procedures.
規格
Detection MethodFluorescence
Excitation/Emission434/539
For Use With (Equipment)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Label or DyeAlexa Fluor Dyes
Product TypeStreptavidin Conjugate (Fluorescent)
Quantity1 mg
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 430
FormSolid
Product LineAlexa Fluor
Unit SizeEach
內容物與存放
Contains 1 vial of streptavidin Alexa Fluor™ 430 conjugate.

Store in freezer (-5 to -30°C) and protect from light.

常見問答集 (常見問題)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

引用資料與參考文獻 (1)

引用資料與參考文獻
Abstract
Detection and quantification of biotinylated proteins using the Storm 840 Optical Scanner.
Authors:Lewis B, Rathman S, McMahon RJ
Journal:J Nutr Biochem
PubMed ID:12770643
'The use of the avidin-biotin interaction is becoming an increasingly common method for the detection of proteins. The use of fluorescence detection with avidin-biotin systems has the potential to greatly increase both the sensitivity and linearity of this type of analysis. In this report, three fluorescent systems were tested for ... More