PBS, pH 7.4

Gibco Phosphate Buffered Saline (PBS), pH 7.4, is a balanced salt solution widely used in various cell culture applications. This product is specifically formulated without calcium, magnesium, and phenol red, making it ideal for a range of laboratory procedures.

Key features

• Without calcium and magnesium—the absence of calcium and magnesium prevents unwanted cell adhesion and aggregation, which is crucial during cell dissociation and flow cytometry
• Without phenol red—the lack of phenol red ensures that there is no interference with optical measurements and prevents potential effects on cell physiology

Applications

• Washing cells before dissociation—PBS is commonly used to rinse cells before enzymatic dissociation, ensuring that chelators are removed from the culture
• Transporting cells or tissue—PBS provides a stable environment for cells or tissues during transportation
• Diluting cells for counting—PBS is used to dilute cell suspensions for accurate counting
• Preparing reagents—PBS serves as a buffer in the preparation of various reagents

Why choose PBS over DPBS

• Simplicity—PBS is a simpler formulation compared to Dulbecco's Phosphate-Buffered Saline (DPBS), which includes additional components like potassium chloride and may be formulated with or without calcium, magnesium, glucose, and pyruvate. This simplicity makes PBS a versatile choice for general cell culture applications.
• Specific applications—PBS is preferred for applications where the presence of potassium or other additives in DPBS might interfere with experimental outcomes

Why choose pH 7.4 over pH 7.2

• Physiological relevance—pH 7.4 is closer to the physiological pH of human blood, making it more suitable for maintaining the natural state of cells during experiments. This pH is particularly important for applications involving mammalian cells.

Why choose Gibco PBS, pH 7.4

• Quality and reliability—manufacture of Gibco PBS, pH 7.4, is cGMP-compliant under the ISO 13485 standard, ensuring high standards of quality and consistency
• Extensive testing—lot release testing of osmolality, pH levels, stability, endotoxins, and sterility ensures the quality of the product
• Proven track record—Gibco cell culture products have a long-standing reputation in the scientific community, with numerous publications citing the use of Gibco PBS, reflecting its widespread acceptance and trust among researchers

Gibco PBS, pH 7.4, is a reliable and high-quality solution for various applications. Its formulation without calcium, magnesium, and phenol red makes it versatile and suitable for specific experimental needs. Choosing Gibco PBS ensures consistency, quality, and support from a trusted brand in the scientific community.