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Nunc™ TripleFlask Treated Cell Culture Flasks
Actual product may vary
Nunc™ TripleFlask Treated Cell Culture Flasks
Nunc™ TripleFlask Treated Cell Culture Flasks

Nunc™ TripleFlask Treated Cell Culture Flasks

Perform large cell mass applications, such as drug screening and scale up, with these cell-culture treated TripleFlask cell culture flasks.
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Catalog NumberSurface TreatmentClosure TypeBarcode
132925Nunclon DeltaFilterYes
Catalog number 132925
HSN 39233090
Price (INR)
17,454.00
CS
Surface Treatment:
Nunclon Delta
Closure Type:
Filter
Barcode:
Yes
Price (INR)
17,454.00
CS

Perform cell culture expansion and experience 500 cm2 total growth area within the footprint of a standard flask using Thermo Scientific Nunc TripleFlask Treated Cell Culture Flasks. Total culture area is achieved by way of the three parallel layers and a straight neck design that works effectively within automation systems. These flasks are subjected to Nunclon Delta treatment, a proprietary cell culture surface treatment that offers maximum adhesion for a broad range of cell types.

Three layers of growth surface provide a total culture area of 500 cm2 in the same footprint as a standard Nunc 175 cm2 flask.

  • Recommended working volume is 200 mL
  • Promotes monolayer adherent cell culture
  • Also available barcoded with large Code 128
  • Nunclon Delta Certificate included in each case for monolayer formation, cloning efficiency, non-cytotoxic, non-pyrogenic, and sterility

Warranty: 90 days

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
Specifications
BarcodeYes
DescriptionNunclon Delta Treated TripleFlask, Filter Cap, Barcoded
Capacity (Metric)200 mL
MaterialPolystyrene
Neck StyleStraight
No. per Case32/Cs.
No. per Pack1 Ea.
Product TypeFlask
ShapeSame external dimensions as the standard Nunc T175 with straight neck
DisposableSingle-use
GraduatedYes
SterilitySterile
Bottom ShapeFlat
Closure TypeFilter
Culture Area500 cm²
Surface TreatmentNunclon Delta
Unit SizeCS

Frequently asked questions (FAQs)

Can I use a Nunc Cell Scraper with a Nunc TripleFlask that has Nunclon Delta surface treatment?

The design of the Nunc TripleFlask does not allow for mechanical scraping. However, cells are easily harvested using protein digesting enzymes, such as trypsin, collagenase, and Pronase or by using non-enzymatic dissociation solutions.
The following is a document link regarding culturing techniques for Nunc TripleFlask:
https://assets.thermofisher.com/TFS-Assets/BID/Technical-Notes/culturing-technique-nunc-tripleflask-technical-note.pdf

What is the recommended working volume of Nunc adherent cell culture products?

This depends largely on the cell line and working conditions (considerations include headspace for gas exchange, supplements used, and optimal feeding schedule, among others) but we do offer guidelines for development purposes (0.3 to 0.5 mL per cm2) . Note that these volumes are approximate and may have to be modified for best performance. T25 Flask = 7 mL, T75 Flask = 25 mL, T175 Flask = 55 mL, T225 Flask = 70 mL, 300cm2 Flask = 100 mL, 500 cm2 TripleFlask = 200 mL, 35 mm round culture dish = 3 mL, 60 mm round culture dish = 5 mL, 100 mm round culture dish = 12 mL, 150 mm round culture dish = 35 mL, 4 (rectangular) well Multidish = 5 mL, 8 (rectangular) well Multidish = 3 mL, 4 (round) well Multidish = 1 mL, 6 well Multidish = 3 mL, 12 well Multidish = 2 mL, 24 well Multidish = 1 mL, 48 well Multidish = 0.5 mL.