Nunc™ Cell Culture Inserts in Carrier Plate Systems

Use up to 24 inserts at a time with Thermo Scientific™ Nunc™ Carrier Plate for Cell Culture Inserts, for individual compartment cultures or cultures with shared media.

Catalog Number	Pore Size	No. of Wells
141086 also known as PA141086	No Inserts	12

Catalog number 141086

also known as PA141086

HSN 39269099

Price (INR)

13,353.00

Pore Size:

No Inserts

No. of Wells:

Price (INR)

13,353.00

The Thermo Scientific™ Nunc™ Carrier Plate system is designed to enable three different hanging height adjustments for the Cell Culture Inserts to accommodate liquid-air-interface culture and various cell feeding protocols. Individually packed, the multi-well Carrier Plate system comes with or without the Cell Culture Inserts.

Features:

Each system includes a standard multi-well cell culture plate, a Carrier Plate, and a lid. System comes with or without Cell Culture Inserts
To adjust insert hanging height in the well, rotate the insert to engage one of the three height-adjusting tabs in the carrier plate
Plates are labeled with alphanumeric well identification
Polycarbonate porous membrane of the insert is cell culture-treated for optimized cell attachment
Inserts come in 3 pore sizes: 0.4, 3, and 8 micron
Plates come in two formats: 12- and 24-well

Warranty: 90 days

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Specifications

MaterialPolycarbonate (Membrane)

Volume (Metric) Working2 mL (Low Hanging Position), 3 mL (Medium Hanging Position), 4 mL (High Hanging Position)

Pore DensityNo Inserts

Pore SizeNo Inserts

TypeCarrier Plate

No. per Pack1

No. per Case4

Dish TypeCarrier Plate only

ColorClear

SterilitySterile

No. of Wells12

Inserts per Plate0

Unit SizeCS

Frequently asked questions (FAQs)

With the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates, what is the distance between the cell culture membrane and the bottom of the multi-well dish?

The membrane is approximately 1.0 mm above the bottom of the dish for the Nunc Polycarbonate Cell Culture Inserts.

Can I observe cells on the cell culture membrane when the Nunc Polycarbonate Cell Culture Insert is inside of a multi-well plate?

Due to the large pore size of the Nunc polycarbonate inserts, spots or holes are observed and they represent the membrane. In this plane of focus, the most visible cells are the ones located on the membrane, whereas the slightly blurred backdrop are the cells on the dish that are in a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of the membrane. However, these cells are visible in culture.

What is the recommended Multidish chamber media volume per carrier plate insert and height position?

Low position: 2 mL (12-well), 1 mL (24-well), Medium position: 3 mL (12-well), 1.5 mL (24-well), High position: 4 mL (12-well), 2 mL (24-well). Some optimization may be necessary for development purposes.

What is the difference between your cell culture membrane inserts and carrier plates?

Standard culture membrane inserts are placed directly into the wells of a Multidish, and the feet at the bottom of the insert maintain a distance of about 1 mm between the membrane and the Multidish culture surface. The “carrier plate” culture membrane product adds a tray between the Multidish and the lid, with a set of hanger brackets at each well. These brackets engage one of three sets of tabs on each insert, to allow for selection between 3 different distances (approximately 1, 3, or 6 mm) between the membrane and the Multidish culture surface. This can allow for more flexibility in experiments, such as distance-dependent interaction between populations or media volume under cultures at the air-media interface.