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Citations & References (19)
Invitrogen™
ViewRNA™ Tissue Assay Blue Module
This VewRNA Tissue Assay Blue Module is designed for use with the ViewRNA Tissue Assay Core Kit. The Core Kit,Read more
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| Catalog Number | No. of Reactions |
|---|---|
| 19932 | 24 Assays |
| 19943 | 96 Assays |
Catalog number 19932
Price (INR)
53,719.00
24 assays
No. of Reactions:
24 Assays
Price (INR)
53,719.00
24 assays
This VewRNA Tissue Assay Blue Module is designed for use with the ViewRNA Tissue Assay Core Kit. The Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. (Detect up to four RNA or microRNA in cryo or FFPE sectioned tissue with ViewRNA Tissue Fluorescence Assay.) Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).
ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.
With ViewRNA Tissue assays, you can achieve:
- Robust, single mRNA molecule detection in tissue sections
- Accuracy and specificity inherent to branched DNA amplification technologies
- Simultaneous detection of two RNA targets
The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
No. of Reactions24 Assays
Product LineViewRNA
Product TypeRNA Tissue Assay Blue Module
Quantity24 Assays
Shipping ConditionWet Ice
Unit Size24 assays
Contents & Storage
• 45 μL Label Probe 6-AP (2°C to 8°C)
• 420 μL Blue Reagent 1°C(2 to 8°C)
• 420 μL Blue Reagent 2°C(2 to 8°C)
• 420 μL Blue Reagent 3°C(2 to 8°C)
• 20 mL Blue Buffer (2°C to 8°C)
• 10 mL AP Stop QT (2°C to 8°C)
• 420 μL Blue Reagent 1°C(2 to 8°C)
• 420 μL Blue Reagent 2°C(2 to 8°C)
• 420 μL Blue Reagent 3°C(2 to 8°C)
• 20 mL Blue Buffer (2°C to 8°C)
• 10 mL AP Stop QT (2°C to 8°C)
Frequently asked questions (FAQs)
Can I purchase the individual components of the ViewRNA Tissue Assay Core Kit and ViewRNA Tissue Assay Blue Module as standalone products?
Label Probe Diluent is provided in the ViewRNA Tissue Assay Core Kit but not in the ViewRNA Tissue Assay Blue Module. Can I purchase this separately?
Does ViewRNA Tissue Assay Blue Module (Cat Nos. 19932, 19943) use the same Fast Blue reagent as used in the earlier Fast Blue Substrate products?
How is ViewRNA Tissue Assay Blue Module (Cat Nos. 19932, 19943) different from the earlier Fast Blue Substrate products?
Citations & References (19)
Citations & References
Abstract
Excessive cytokine response to rapid proliferation of highly pathogenic avian influenza viruses leads to fatal systemic capillary leakage in chickens.
Journal:PLoS One
PubMed ID:23874602
Highly pathogenic avian influenza viruses (HPAIVs) cause lethal infection in chickens. Severe cases of HPAIV infections have been also reported in mammals, including humans. In both mammals and birds, the relationship between host cytokine response to the infection with HPAIVs and lethal outcome has not been well understood. In the
Distribution of serotonin receptor 5-HT6 mRNA in rat neuronal subpopulations: A double in situ hybridization study.
Journal:
PubMed ID:26424380
'The 5-HT6 receptor (5-HT6R) is almost exclusively expressed in the brain and has emerged as a promising target for cognitive disorders, including Alzheimer''s disease. In the present study, we have determined the cell types on which the 5-HT6R is expressed by colocalizing 5-HT6R mRNA with that of a range of
Chemokine (C-C motif) receptor 5 is an important pathological regulator in the development and maintenance of neuropathic pain.
Journal:
PubMed ID:24589480
'The chemokine family has been revealed to be involved in the pathogenesis of neuropathic pain. In this study, the authors investigated the role of chemokine (C-C motif) ligand 3 and its receptors chemokine (C-C motif) receptor 1 and chemokine (C-C motif) receptor (CCR) 5 in neuropathic pain.'
Metabolic regulator LKB1 is crucial for Schwann cell-mediated axon maintenance.
Journal:
PubMed ID:25195104
Schwann cells (SCs) promote axonal integrity independently of myelination by poorly understood mechanisms. Current models suggest that SC metabolism is critical for this support function and that SC metabolic deficits may lead to axonal demise. The LKB1-AMP-activated protein kinase (AMPK) kinase pathway targets several downstream effectors, including mammalian target of
Characterization of target mRNA reduction through in situ RNA hybridization in multiple organ systems following systemic antisense treatment in animals.
Journal:
PubMed ID:24161045
Advances in the medicinal chemistry of antisense oligonucleotide drugs have been instrumental in achieving and optimizing antisense activity in cell types other than hepatocytes, the cell type that is most sensitive to antisense effects following systemic treatment. To broadly characterize the effects of antisense drugs on target messenger RNA (mRNA)