Opti-MEM™ I Reduced Serum Medium

Name: Opti-MEM™ I Reduced Serum Medium
SKU: 31985054

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine SerumRead more

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Catalog Number	Quantity
31985054 also known as 31985-054	10 x 500 mL
31985047 also known as 31985-047	500 mL

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Catalog number 31985054

also known as 31985-054

Price (INR)

Quantity:

10 x 500 mL

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Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.

This Opti-MEM™ I is modified as follows:

With

• L-glutamine

• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Specifications

Cell TypeMammalian Cells

Concentration1 X

Culture EnvironmentCO₂

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Osmolality280 - 320 mOsm/kg

Product LineGibco, Opti-MEM

Product TypeReduced-Serum Medium

Quantity10 x 500 mL

Shelf Life18 Months

ClassificationAnimal Origin

Culture TypeMammalian Cell Culture

FormLiquid

SterilitySterile-filtered

With AdditivesGlutamine, HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate

pH7 to 7.4

Unit SizeEach

Contents & Storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 18 months from date of manufacture.

Frequently asked questions (FAQs)

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I noticed that Opti-MEM I Reduced Serum Medium does not contain any serum. Why do you then refer to it as "Reduced Serum Medium"?

Opti-MEM I Reduced Serum Medium is a serum-free medium. The reason why it is called “reduced-serum medium” is because you only need to supplement this medium with 1-5% FBS instead of the standard 10% FBS.

Opti-MEM I Reduced Serum Medium can be used as is (without serum) for transfection of cells. However, for long-term cell culture, you need to add serum to the medium to keep cells healthy.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.