GeneAmp™ 10X PCR Buffer II & MgCl2
GeneAmp&trade; 10X PCR Buffer II &amp; MgCl<sub>2</sub>
Applied Biosystems™

GeneAmp™ 10X PCR Buffer II & MgCl2

Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
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Catalog NumberQuantity
43798782 x 75 mL
N80800101 x 1.5 mL
N80801306 x 1.5 mL
Catalog number 4379878
Price (INR)
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Quantity:
2 x 75 mL
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Applied Biosystems™ GeneAmp™ 10X PCR Buffer II is optimized for use with AmpliTaq™ DNA Polymerase, resulting in robust PCR amplification. The provided MgCl2 solution offers versatility for optimizing PCR.
  • Optimized for use with AmpliTaq™ DNA Polymerase, offering maximum PCR performance
  • Available in several convenient sizes
  • Separate MgCl2 solution offers versatility

GeneAmp™ 10X PCR Buffer II, when diluted 1:10, provides the optimal pH and ionic strength for PCR amplification. GeneAmp™ 10X PCR Buffer II is magnesium ion free. A separate MgCl2 solution is provided. When used together, GeneAmp™ 10X PCR Buffer II and MgCl2 solution offer more versatility in optimizing the magnesium ion concentration to achieve optimal PCR amplification with any specific set of primers and DNA template.

Note: See user's manual or package insert for limited label license and trademark information.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)1X
PolymeraseAmpliTaq DNA Polymerase
Product TypePCR Buffer
Quantity2 x 75 mL
Reaction FormatKit
Shipping ConditionWet Ice
Volume150 mL
Concentration10X
For Use With (Application)Standard PCR
Unit SizeEach
Contents & Storage
• GeneAmp 10X PCR Buffer II, 2 x 75 mL
• MgCl2, 2 x 75 mL

Store at -15°C to -25°C.

Frequently asked questions (FAQs)

What are the differences between GeneAmp 10X PCR Buffer I, PCR Buffer II and PCR Gold Buffer, and what are the formulations of each buffer?

The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been carefully optimized for use with the AmpliTaq Gold enzyme. GeneAmp 10X PCR Buffer I contains Mg; Geneamp 10X PCR Buffer II and Geneamp 10X PCR Gold Buffer do not have Mg in them, but come with a separate vial of 25 mM MgCl2 so that you can titrate the Mg in your PCR reaction.

Buffer formulations:
Buffer I (500 mM KCl, 100 mM Tris-HCl, pH 8.3, 15 mM MgCl2, 0.01% (w/v) gelatin)
Buffer II (500 mM KCl, 100 mM Tris-HCl, pH 8.3), with separate tube of 25 mM MgCl2
Gold Buffer (500 mM KCl, 150 mM Tris-HCl pH 8.0), with separate tube of 25 mM MgCl2

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.