Ambion™ mirVana™ miRNA Inhibitor, let-7c positive control
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Invitrogen™

Ambion™ mirVana™ miRNA Inhibitor, let-7c positive control

miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules andRead more
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Catalog NumberQuantity
446408120 nmol
44640805 nmol
Catalog number 4464081
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Quantity:
20 nmol

miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.

We have now released the second generation of miRNA inhibitors, mirVana™ miRNA Inhibitors, which:

  • Display better potency than lead competitor's product
  • Offer maximum and consistent effect in vitro at low miRNA inhibitor concentration
  • Target the most updated Sanger content
  • Can be used in vitro and in vivo, offering consistency throughout your entire research project
  • Are non-toxic

Positive and negative controls are also available for use along with mirVana™ miRNA Inhibitors in miRNA down-regulation experiments.

mirVana™ miRNA Inhibitor let-7c Positive Control

The mirVana™ miRNA Inhibitor let-7c Positive Control is intended for use as a positive control for mammalian cell culture experiments using mirVana™ miRNA Inhibitors. Endogenous let-7c miRNA has been shown to negatively regulate HMGA2 mRNA in cultured cells. HMGA2 is a ubiquitously expressed, nonhistone, chromatin protein that can modulate gene expression through changes in chromatin architecture. let-7c miRNA down-regulates levels of HMGA2 mRNA. When transfected into human and mouse cell lines, let-7c miRNA inhibitor blocks endogenous let-7c miRNA, resulting in increased levels of HMGA2 mRNA. Thus, let-7c miRNA inhibitor activity can be monitored in human or mouse cells using real-time reverse transcription PCR (RT-PCR) to detect HMGA2 mRNA.

First Generation Inhibitor Controls

Our first-generation miRNA inhibitors (and controls), Ambion™ Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Inhibitors. These first-generation inhibitors are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Inhibitors and their corresponding controls.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)miRNA & Non-Coding RNA Analysis
Label or DyeUnconjugated
Product LinemirVana
Product TypemiRNA Inhibitor
Purification MethodPurified
Quantity20 nmol
Shipping ConditionRoom Temperature
Control TypePositive Control
RNAi TypemiRNA Inhibitor
Unit SizeEach
Contents & Storage
Store at or below -20°C. Do not store in a frost-free freezer.

Frequently asked questions (FAQs)

How can I quantify endogenous miRNA inactivation by mirVana inhibitors?

Using TaqMan miRNA assays, you can measure the level of miRNA of interest. If the miRNA is bound to the synthetic anti-miRs and/or mirVana inhibitors transfected, the duplex should not be detected, indicating efficient miRNA inactivation. Please note, this only works when total RNA sample isolated is not heat denatured when RT primer is added, as heat denaturation will result in complex dissociation and subsequent detection of microRNA by TaqMan miRNA assay even when cells have been transfected properly. Please see the protocol for the TaqMan MicroRNA Cells-to-CT kit (Cat. No. 4391848) for more details.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How do you introduce mirVana miRNA mimics or inhibitors into cells?

Mimics and inhibitors can be transfected or electroporated into cells using parameters similar to those used for siRNA.

Using mirVana miRNA Mimics- For the following applications, use a cell line that expresses relatively low levels of the mature miRNA of interest.
- Cell density at the time of transfection. In general, 30-70% confluency is recommended.
- Transfection method (traditional or reverse transfection).
- Length of exposure of cells to transfection agent-RNA oligonucleotides complexes.
For many miRNA mimics, maximal activity is achieved after 24 hours, but biological assays may be performed 24-72 hours posttransfection. If you maintain your transfected cells longer than 24 hours, we suggest replacing the existing medium with fresh medium at 24 hours posttransfection, for greater viability of the cells.

Using mirVana miRNA Inhibitors to analyze endogenous miRNA function
For the following applications, use a cell line that expresses relatively high levels of the mature miRNA of interest.
General transfection starting points
mirVana miRNA Mimics and Inhibitors typically work best when transfected at a final concentration of 3-30 nM. Optimization experiments might include a concentration range of 1-100 nM. To increase accuracy and reproducibility when preparing transfection complexes, prepare a dilution of your stock oligonucleotides, and pipet a higher volume of diluted stock.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the turnaround time for your mirVana miRNA Mimic and Inhibitors?

The turnaround time varies depending on the level of purification and whether it is a custom order or not. Please see the approximate timetable below:

Standard purification (5 and 20 nmol), 4 business days
HPLC/in vivo ready (250 nmol), 15 business days
Custom mirVana Mimics and Inhibitors, 15 business days

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I would like to create a custom mirVana mimic and inhibitor. Can I do this?

Yes, please provide us with your unpublished miRNA sequence and we can synthesize a corresponding mirVana mimic or inhibitor by clicking on this link (www5.invitrogen.com/custom-genomic-products/tools/mirna/).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Can I get the sequence for the mirVana Mimics and Inhibitors negative control?

Negative control sequences require a confidential disclosure agreement while target sequences require prior proof of purchase for release.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

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