Plates and Modules with Covalent Binding Surfaces
Actual product may vary
Plates and Modules with Covalent Binding Surfaces
Plates and Modules with Covalent Binding Surfaces
Plates and Modules with Covalent Binding Surfaces

Plates and Modules with Covalent Binding Surfaces

Reduce the amount of reagents used in the lab by using these plates and modules with covalent binding surfaces.
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Catalog NumberColorVolume (Metric) WorkingProduct TypeSurface Treatment
478042Clear100 μLSolid Module F8 (x12) in FrameCovalent Binding
436006Clear100 μLMicroplateAmine
436007White100 μLSolid plate 96-WellCovalent Binding
436008Black100 μLMicroplateAmine
436013Clear100 μLMicroplateAmine
436023Clear100 μLMicroplateAmine
Catalog number 478042
HSN 39269099
Price (INR)
52,057.00
Case
Color:
Clear
Volume (Metric) Working:
100 μL
Product Type:
Solid Module F8 (x12) in Frame
Surface Treatment:
Covalent Binding
Price (INR)
52,057.00
Case

Reduce the amount of reagents used in the lab with Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces.

Covalent Surfaces (Thermo Scientific™ CovaLink™ and Immobilizer Amino)

  • Minimal leaching
  • Withstands vigorous washing
  • Coating with lower amounts of reagent may be possible
  • Control of orientation

Immobilizer is a trademark of Exiqon A/S, Vedbaek, Denmark. The product is produced under license from Exiqon A/S and covered by EP 08 20483 and foreign application and patents.

Immobilizer is a trademark of Exiqon A/S, Vedbaek, Denmark. The product is produced under license from Exiqon A/S and covered by EP 08 20483 and foreign application and patents.
Specifications
FormatStandard
Detection MethodColorimetric
Array8 x 12
Packaging30 Cases
ColorClear
Product TypeSolid Module F8 (x12) in Frame
MaterialPolystyrene
Volume (Metric) Well400 μL
No. of Wells96
Volume (Metric) Working100 μL
Surface TreatmentCovalent Binding
Total Volume (Metric) Well400 μL
Well ShapeRound, Flat Bottom (F-well)
No. per Case30
No. per Pack5
Plate BlockingUnspecified
Target MoleculePeptides
Unit SizeCase

Frequently asked questions (FAQs)

What length of peptide is ideal for binding to the Covalink NH surface, and what are the detection limitations?

We have tested and found that a three amino acid peptide (Pro, Leu, Gly) cannot be detected when passively adsorbed on the MaxiSorp surface. However, this peptide can be detected when covalently immobilized using CovaLink NH modules and plates. Using covalent immobilization of small peptide residues, one can obtain a better orientation of the molecule and reduced problems with antibody recognition of the peptide due to masking of the epitope.

Are your Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules compatible with all Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates?

Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

What are some recommendations for improving binding efficiency of proteins and DNA (nucleic acids) using Nunc CovaLink surface modules?

We recommend using a freshly made methylimidazole and carbodiimide condensing agents for optimal covalent binding of both proteins and DNA (nucleic acids). The binding efficiency of single-stranded oligo (a 5' phosphorylated end of a single-stranded oligomer binds with a phosphoramidate bond) is about 8 - 10% with a typical 25 base oligo.

When should I use Nunc CovaLink surface modules?

We recommend Nunc CovaLink surface modules for use with molecules that adsorb with difficulty to a traditional surface. Due to the covalent binding, very thorough washing is possible, which allows for orientation of the molecule and better recognition by the detection molecule.