Search

  • Order Status
  • Quick Order
Pierce™ Universal Nuclease for Cell Lysis PROMO
Actual product may vary
Citations & References (1)

Pierce™ Universal Nuclease for Cell Lysis PROMO

Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.
Have Questions?
Catalog number 88700
also known as 88700DRAK
HSN 90279090
Price (INR)
22,401.00
Pack
Price (INR)
22,401.00
Pack

Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has very high specific activity (100-fold greater than DNase I) and has increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99% pure enzyme, is free of any measurable protease activity and is supplied at a concentration of 250 U/μL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease by EMD Merck™.

Highlights:

  • Broad spectrum – degrades all forms of DNA and RNA
  • Highest-quality enzyme – nuclease is at least 99% pure, as tested by SDS-PAGE
  • Robust activity – 100-fold greater specific activity than DNase I
  • Versatile – can be used with a wide variety of cell lysis reagents

Recommended for:

Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts; Remove DNA and RNA from recombinant protein preparations prior to downstream processing

Storage Requirements

Store in a cool, dry, well-ventilated area, protected from direct sunlight

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormulationAt least 99% pure enzyme at 250 U/uL in 50% glycerol (v/v), 20 mM NaCl, 2 mM MgCl2, 20 mM Tris, pH 8.0
Product TypeCell Lysis Enzyme
FormLiquid
Product LinePierce
Reagent TypeEnzyme for Cell Lysis
EnzymeNuclease
Sufficient For200 mL Lysate
Quantity5 kU
Unit SizePack
Contents & Storage
Store in a cool, dry, well-ventilated area, protected from direct sunlight.

Frequently asked questions (FAQs)

At which concentration/dilution should the Pierce Universal Nuclease for Cell Lysis (Cat. No. 88700) be used, and what is the suggested incubation time and incubation temperature?

We would suggest the following procedure (note that you should determine yourself empirically the optimal incubation time, incubation temperature and also dilution): Please add 25 U or 0.1 µL Pierce Universal Nuclease for Cell Lysis (Cat. No. 88700) to 1 mL of cell lysate (dilution 1:10,000) and incubate at room temperature for 10-15 minutes. If you want to dilute the Nuclease for facilitating pipetting, please do this with Tris- or PBS-buffer at pH 7-8.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

After cell lysis, my mass spectrometry sample is very viscous and difficult to pipette. How do I reduce the sample viscosity?

High sample viscosity after lysis is due to release of DNA from the nucleus. Sonication or addition of a nuclease such as the Pierce Universal Nuclease (Cat. No. 88700, 88701, or 88702) can be used to degrade DNA and reduce sample viscosity.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can you explain how the B-PER Bacterial Protein Extraction Reagent lyses cells?

The B-PER Reagent solution contains a proprietary, mild, non-ionic detergent in 20 mM Tris-HCl, pH 7.5. It effectively disrupts cells and solubilizes native or recombinant proteins without denaturation. The reagent creates holes in the cell membrane that will leak out cytosolic proteins. The sample may become very viscous when the bacterial chromosome is released. We recommend adding DNAse I (Cat. No. 90083) to the reagent to reduce viscosity. For better lysis efficiency and if there are inclusion bodies, we recommend adding Lysozyme (Cat. No. 90082) to the reagent. Alternatively, you may purchase the B-PER Bacterial Protein Extraction Reagent with Enzymes Kit (Cat. No. 90078 or 90079) that includes the B-PER Bacterial Protein Extraction Reagent, DNase I, and Lysozyme.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (1)

Citations & References
Abstract
p53 is active in murine stem cells and alters the transcriptome in a manner that is reminiscent of mutant p53.
Authors:Yan H, Solozobova V, Zhang P, Armant O, Kuehl B, Brenner-Weiss G, Blattner C
Journal:
PubMed ID:25719246
Since it was found that p53 is highly expressed in murine embryonic stem cells, it remained a mystery whether p53 is active in this cell type. We show that a significant part of p53 is localised in the nucleus of murine embryonic stem cells and that the majority of this ... More