GeneChip™ Human Genome U133A 2.0 Array

Name: GeneChip™ Human Genome U133A 2.0 Array
SKU: 900468

The GeneChip™ Human Genome U133A 2.0 Array is a single array representing 14,500 well-characterized human genes that can be usedRead more

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Catalog Number	Number of Arrays
900468	6 arrays
900471	2 arrays
900469	30 arrays

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Catalog number 900468

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Number of Arrays:

6 arrays

The GeneChip™ Human Genome U133A 2.0 Array is a single array representing 14,500 well-characterized human genes that can be used to explore human biology and disease processes.

The GeneChip™ Human Genome U133A 2.0 Array is a single array representing 14,500 well-characterized human genes that can be used to explore human biology and disease processes. New design and reduced feature size mean that you can use smaller sample volumes than the previous HG™U133A Array without compromising performance.

•Provides coverage of well-substantiated genes in the transcribed human genome on a single array
•Analyzes the expression level of 18,400 transcripts and variants, including 14,500 well-characterized human genes
•Comprised of more than 22,000 probe sets and 500,000 distinct oligonucleotide features
•Use the Power of the Probe Set and get multiple independent measurements for each transcript that deliver the greatest accuracy and reproducibility of any microarray platform
•All probe sets represented on the GeneChip™ Human Genome U133A Array are identically replicated on the GeneChip Human Genome U133A 2.0 Array

Array Profile
Sequences used in the design of the array were selected from GenBank™, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then were refined by analysis and comparison with a number of other publicly available databases including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).

Instrument and Software Requirements
•GeneChip™ Scanner 3000, enabled for High-Resolution Scanning*
•GeneChip™ Command Console™ Software (AGCC) including the GeneChip™ Scanner 3000 High-Resolution Scanning Patch

*GeneChip Scanner 3000 High-Resolution Update is standard on all instruments shipped starting in September 2003 with serial number series 502. Previous versions, serial number series 501, will require the 00-0110 GeneChip Scanner 3000 High-Resolution Update to be installed.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product LineGeneChip

Quantity6 arrays

TypeHuman Genome U133A 2.0 Array

ArrayTranscriptome Profiling

FormatArray Cartridge

Number of Arrays6 arrays

SpeciesHuman

Unit Size6 arrays

Frequently asked questions (FAQs)

Why is a set of 100 probes duplicated on the GeneChip Human Genome U133A 2.0 Array?

The intended use of the 100 probe sets is to allow users to normalize data across the chip. These 100 probe sets are considered normalization controls. They were found to have consistent levels of expression across multiple tissue types and treatments.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What does the "_x_at" extension represent in the HG-U133 probe set name?

Occasionally, it is not possible to select either a unique probe set or a probe set with all probes common among multiple transcripts ("_s_at" ). In such cases, similarity criteria are suspended, and the resulting probe set name is appended with the "_x_at" extension. These probe sets contain some probes that are identical, or highly similar, to unrelated sequences. These probes may cross-hybridize in an unpredictable manner with sequences other than the main target. Data generated from these probe sets should be interpreted with caution, due to the likelihood that some of the signal is from transcripts other than the one being intentionally measured.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What does the "_s_at" extension represent in the HG-U133 probe set name?

The primary goal in probe set selection is to select a probe set unique to a single transcript or common among a small set of similar transcript variants. A probe set name is appended with the "_s_at" extension when all the probes exactly match multiple transcripts. The probe set selection process generally favors probe sets measuring fewer transcripts. Probe sets with common probes among multiple transcripts (the "_s_at" probe sets), are frequent and are to be expected, due to alternative polyadenylation and alternative splicing. In most cases, "_s_at" probe sets represent transcripts from the same gene, but the same probe set can sometimes also represent transcripts from homologous genes. One transcript may be represented by both a unique and an "_s_at" probe set when the transcript variation is sufficient.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How do I export a CEL file from GCOS as a text file?

There are two ways to export the CEL file from GCOS v1.4 as a text file.

1. In GCOS 1.4 use GCOS Manager to navigate to the CEL file in the process database. Highlight the CEL file and right click to export the data from the process tab. You may want to change the data location where the export will be stored before you start.
2. In GCOS 1.4 use the find feature in GCOS Manager to find the CEL files and export those selected as a text file. This approach allows export of more than one experiment at a time.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Is it possible to compare different array types (e.g., HG-U133A and HG-U133A 2.0) in GeneChip Command Console (GCC)?

Command Console cannot generate a comparison analysis between different array types.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

View all GeneChip™ Human Genome U133A 2.0 Array, 6 arrays FAQs