GeneChip™ CHO Gene 2.0 ST Array
GeneChip™ CHO Gene 2.0 ST Array
Applied Biosystems™

GeneChip™ CHO Gene 2.0 ST Array

Comprehensive designThe Chinese hamster ovary (CHO) cell lines were introduced in the 1960-s and has become an extremely important toolRead more
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Catalog NumberNumber of Arrays
9021306 arrays
90213130 arrays
Catalog number 902130
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Number of Arrays:
6 arrays
Comprehensive design
The Chinese hamster ovary (CHO) cell lines were introduced in the 1960-s and has become an extremely important tool for biomedical research biopharmaceutical manufacturing. Their rapid growth, stable gene expression profiles, and high protein production make them idea for both research studies and for the production of therapeutic proteins.

To help further your research and improve manufacture yield, we have introduced GeneChip™ CHO Gene 2.0 ST Array, with an expression array design that provides the highest coverage of the transcribed genome. We use a comprehensive collection of information sources to design probes that interrogate up to 23 unique sequences of each transcript. This design strategy provides you with the ability to evaluate whole-transcriptome gene expression at the gene and exon levels, which allows the study of transcript variants and alternative splicing events.

This unique array design is based on the most recent genome content and offers the high probe coverage of every transcript measured by this array. The high transcript coverage of this array design yields accurate detection for genome-wide transcript expression changes and provides higher resolution and accuracy than other classical 3'-biased microarray solutions on the market. The whole-transcriptome analysis approach enables researchers to detect multiple transcript isoforms from a given gene, including those that could be missed using a 3'-biased expression design, such as splice variants, non-polyadenylated transcripts, transcripts with alternative polyadenylation sites, and truncated transcripts.

Key benefits
• Whole-transcript analysis the capture of transcript isoforms you may miss with 3'-biased expression designs
• Comprehensive transcriptome coverage provides the best opportunity to discover interesting biology:
   - >21,000 protein coding transcripts
   - >19,000 Entrez genes
• Measure alternative splicing events/transcript variants with probes designed to maximize coverage of exons
• Reproducible: Signal correlation coefficient ≥0.99

Content profile
The tables below summarize the array content and data sources used to design GeneChip CHO Gene 2.0 ST Array.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineGeneChip
Quantity6 arrays
TypeCHO Gene 2.0 ST Array
ArrayTranscriptome Profiling
FormatArray Cartridge
Number of Arrays6 arrays
SpeciesChinese Hamster
Unit SizeEach

Frequently asked questions (FAQs)

What is contained in the tab-delimited format of the GeneChip probe sequence download file?

The tab-delimited probe sequence file contains the following information:
-Probe Set Name
-Probe X: The X coordinate of the probe sequence on the GeneChip probe array.
-Probe Y: The Y coordinate of the probe sequence on the GeneChip probe array.
-Probe Interrogation Position: The base position on the consensus/exemplar sequence where the central base of the probe aligns, which is the 13th base of a 25mer probe.
-Probe Sequence: The 25-base perfect match sequence.
-Target Strandedness: The sense/antisense orientation of the target sequence that can hybridize with the probe sequence.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the NetAffx Analysis Center?

The NetAffx Analysis Center used to contain information and files regarding array content, probe sets, and functional annotations. However, the NetAffx Analysis Center has been retired. Much of the content provided by the NetAffx Analysis Center can now be accessed on the arrays’ thermofisher.com product pages or by contacting Technical Support (techsupport@thermofisher.com).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Are pseudogene databases included in the design of expression arrays?

Pseudogene databases were not included in the design of expression arrays.

How long can I store labeled cDNA when working with expression microarrays?

Labeled material can be stored for 2 weeks at -20 degrees C.

What is an Event Score in TAC 4.0 Software?

TAC 4.0 includes two algorithms for identifying alternative splicing events: the TAC 2.0 algorithm and the new EventPointer. Algorithmic determination of alternate splicing remains a challenging problem. TAC 4.0 supports two different approaches that have different sets of strengths and weaknesses. After considerable testing, the new TAC 4.0 “'Event Score” leverages both previous TAC 2.0 event estimation score and Event Pointer p-value and sorts the most likely alternative splicing events to the top. Of course, the TAC 2.0 event score and EventPointer p-values remain individually available.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.