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Citations & References (18)
Invitrogen™
ATTO-TAG™ FQ Derivatization Reagent (FQ; 3-(2-Furoyl)quinoline-2-Carboxaldehyde)
The ATTO-TAG FQ reagent allows the ultrasensitive detection of primary amines. This reagent is available as a standalone product orRead more
| Catalog Number | Quantity |
|---|---|
| A10192 | 10 mg |
Catalog number A10192
Price (INR)Request A Quote
-
Quantity:
10 mg
The ATTO-TAG FQ reagent allows the ultrasensitive detection of primary amines. This reagent is available as a standalone product or as a component of an amine-derivatization kit (A-2334). Because ATTO-TAG FQ is nonfluorescent in the unreacted state and reacts specifically with amines to form highly fluorescent conjugates, it is particularly well-suited to the derivatization of peptides, amino acids, aminated sugars and other amine-containing molecules for detection by capillary electrophoresis with laser-induced fluorescence (LIF). Recently, researchers described a simple method in which the ATTO-TAG FQ reagent was used to derivatize very dilute solutions (10-8 M) of peptides.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
For Use With (Application)Detection of primary amines
Label or DyeATTO-TAG FQ
Quantity10 mg
Reactive MoietyAldehyde
Shipping ConditionRoom Temperature
TargetPrimary Amine
FormSolid
Product LineATTO-TAG
TypeDerivatization Kits and Reagents
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (18)
Citations & References
Abstract
Identification of proteins in single-cell capillary electrophoresis fingerprints based on comigration with standard proteins.
Journal:Anal Chem
PubMed ID:14570203
'In the previous paper in this Journal, we reported the use of capillary sieving electrophoresis to characterize proteins expressed by single cancer cells at specific phases in the cell cycle. Analysis of the data revealed one component with cell cycle-dependent changes in expression at the 99% confidence limit. However, the
Kinetics and apparent activation energy of the reaction of the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde with ovalbumin.
Journal:J Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID:12880858
'Incomplete labeling of proteins by a derivatizing reagent usually results in the formation of a large number of products, which can produce unacceptable band broadening during electrophoretic analysis. In this paper, we report on the reaction of the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde (FQ) with the lysine residues of ovalbumin. Mass spectrometry
Solid-phase fluorescent labeling reaction of picomole amounts of insulin in very dilute solutions and their analysis by capillary electrophoresis.
Journal:Electrophoresis
PubMed ID:7588523
'The fluorescent labeling of peptides at concentrations as low as 10(-8) M can be achieved by using a solid-phase reactor. Using oxidized insulin chain B as a test peptide, we demonstrate the use of an Immobilon CD membrane to capture and preconcentrate peptides. Insulin chain B can then be labeled
MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection.
Journal:J Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID:12880861
'MitoTracker Green (MTG) is a mitochondrial-selective fluorescent label commonly used in confocal microscopy and flow cytometry. It is expected that this dye selectively accumulates in the mitochondrial matrix where it covalently binds to mitochondrial proteins by reacting with free thiol groups of cysteine residues. Here we demonstrate that MTG can
Analysis of aminophospholipid molecular species by methyl-beta-cyclodextrin modified micellar electrokinetic capillary chromatography with laser-induced fluorescence detection.
Journal:Electrophoresis
PubMed ID:12207317
'Micellar electrokinetic capillary chromatography (MEKC) with laser-induced fluorescence detection is used for the analysis of three classes of aminophospholipids: phosphatidylethanolamine (PE), phosphatidylserine (PS), and lysophosphatidylethanolamine (LPE) molecular species. 3-(2-Furoyl) quinoline-2-carboxaldehyde (FQ), a fluorogenic dye, was employed for labeling of these phospholipids. The FQ-labeled lipid species were then separated by sodium