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Citations & References (233)
Invitrogen™
Bis-ANS (4,4'-Dianilino-1,1'-Binaphthyl-5,5'-Disulfonic Acid, Dipotassium Salt)
Bis-ANS is superior to 1-8-ANS as a probe for nonpolar cavities in proteins, often binding with an affinity that isRead more
| Catalog Number | Quantity |
|---|---|
| B153 | 10 mg |
Catalog number B153
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Quantity:
10 mg
Bis-ANS is superior to 1-8-ANS as a probe for nonpolar cavities in proteins, often binding with an affinity that is orders-of-magnitude higher. Anilinonaphthalene sulfonates (ANS) are essentially nonfluorescent in water, only becoming appreciably fluorescent when bound to membranes. This property makes it a sensitive indicator of protein folding, conformational changes and other processes that modify the exposure of the probe to water.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Label TypeOther Label(s) or Dye(s)
Molecular FormulaC32H22K2N2O6S2
Product LineMolecular Probes
Quantity10 mg
Recommended StorageStore at room temperature and protect from light.
Shipping ConditionRoom Temperature
Sub Cellular LocalizationCell Membranes & Lipids
Physical FormSolid
Product TypeProtein Folding Indicator
Unit SizeEach
Citations & References (233)
Citations & References
Abstract
Interaction of 1,1'-bi(4-anilino)naphthalene-5,5'-disulfonic acid with alpha-crystallin.
Journal:J Biol Chem
PubMed ID:9535881
'The hydrophobic sites in alpha-crystallin were evaluated using a fluorescent probe 1,1''-bi(4-anilino)naphthalenesulfonic acid (bis-ANS). Approximately one binding site/subunit of alpha-crystallin at 25 degrees C was estimated by equilibrium binding and Scatchard analysis (Kd = 1.1 microM). Based on fluorescence titration, the dissociation constant was 0.95 microM. The number of bis-ANS
Plurality of protein conformations of ribulose-1,5-bisphosphate carboxylase/oxygenase monomers probed by high pressure electrophoresis.
Journal:J Biol Chem
PubMed ID:7902356
'We used hydrostatic pressure in the range of 1 to 2 kbar, coupled with polyacrylamide gel electrophoresis, to investigate the properties of monomers of dimeric ribulose bisphosphate carboxylase/oxygenase. At temperatures below -5 degrees C or pressures above 1.5 kbar, only a diffuse band with low electrophoretic mobility was observed, which
An operator-induced conformational change in the C-terminal domain of the lambda repressor.
Journal:J Biol Chem
PubMed ID:1532575
'4,4''-bis(1-anilino-8-naphthalenesulfonic acid (Bis-ANS), an environment-sensitive fluorescent probe for hydrophobic region of proteins, binds specifically to the C-terminal domain of lambda repressor. The binding is characterized by positive cooperativity, the magnitude of which is dependent on protein concentration in the concentration range where dimeric repressor aggregates to a tetramer. In this
Exploiting new potential targets for anti-hepatitis B virus drugs.
Journal:Curr Drug Targets Infect Disord
PubMed ID:14529356
'Based on the recent studies of HBV strains with different replication efficiency, several new potential targets for anti-HBV replication have been presented. These include the viral and cellular regulatory factors associated with HBV replication and the process for encapsidation of viral genome and budding into endoplasmic reticulum (ER). A putative
Detection of disulfide bonds in bovine brain tubulin and their role in protein folding and microtubule assembly in vitro: a novel disulfide detection approach.
Journal:Biochemistry
PubMed ID:11467944
'Cysteine residues in tubulin are actively involved in regulating ligand interactions and microtubule formation both in vivo and in vitro. These cysteine residues are sensitive reporters in determining the conformation of tubulin. Although some of the cysteines are critical in modulating drug binding and microtubule assembly, it is not clear