Anaerobe Basal Broth (Dehydrated)
Anaerobe Basal Broth (Dehydrated)
Thermo Scientific™

Anaerobe Basal Broth (Dehydrated)

Cultivate anaerobic microorganisms, particularly Bacteroides spp. and other fastidious anaerobes with this medium.

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CM0957B500 g
Catalog number CM0957B
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500 g

Cultivate anaerobic microorganisms, particularly Bacteroides spp. and other fastidious anaerobes with Thermo Scientific™ Oxoid™ Anaerobe Basal Agar (Dehydrated). Anaerobe Basal Broth is formulated from a range of nutrients which have been selected to optimize the recovery and growth of the majority of anaerobic organisms of clinical importance.

Anaerobe Basal Agar contains peptones, carefully selected to support good growth of anaerobic bacteria and yeast extract as a vitamin source. Starch is present to absorb any toxic metabolites1. Sufficient arginine is added to ensure the growth of Eubacterium lentum2, while haemin and vitamin K are growth factors required by many Bacteroides species3. Haemin is also required by Porphyromonas species. Sodium succinate improves the growth of Prevotella melaninogenica and Bacteroides species4. Sodium pyruvate is added as an energy source for asaccharolytic cocci such as Veillonella. It also acts similarly to catalase and degrades traces of hydrogen peroxide, which may be produced by the action of molecular oxygen on media components5. L-cysteine hydrochloride and dithiothreitol are reducing agents, and cysteine has also been shown to stimulate the growth of some anaerobes6.

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General References:

  1. Ajello GW. Geely JC. Hayes PS. et al. Trans-isolate medium: a new medium for primary culturing and transport of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. J. Clin. Micro. 1984:20:55-8.
  2. Sperry JF. Wilkins TD. Arginine, a growth-limiting factor for Eubacterium lentum. J. Bacteriol. 1976:127:780-4.
  3. Gibbons RJ. und MacDonnald JB. Haemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J. Bact. 1960:80:164-170.
  4. Lev M. Keudell KC. und Milford AF. Succinate as a growth factor for Bacteroides melaninogenicus. J. Bact. 1971:108:175-8.
  5. Neilson PA. Role of reduced sulphur compounds in nutrition of Proprionobacterium acnes. J. Clin. Micr. 1983:17:276-9.
  6. Shanson DC. und Singh J. Effect of adding cysteine to brain-heart infusion broth on the isolation of Bacteroides fragilis from experimental blood cultures. J. Clin. Path. 1981:34:221-3.
Specifications
DescriptionAnaerobe Basal Broth
For Use With (Application)Healthcare
Quantity500 g
FormPowder
Product TypeMicrobiology Media
Unit SizeEach